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1、BiochemicalSystematicsandEcology48(2013)34–41ContentslistsavailableatSciVerseScienceDirectBiochemicalSystematicsandEcologyjournalhomepage:www.elsevier.com/locate/biochemsysecoGeneticandphytochemicaldiversitiesofCynomoriumsongaricumRupr.inNorthwestChinaindicate
2、dbyISSRmarkersandHPLC-fingerprintingGuang-DaLiu,Gui-LinChen*,WeiLi,Chun-XingLiCollegeofLifeScience,InnerMongoliaUniversity,Hohhot010021,ChinaarticleinfoabstractArticlehistory:CynomoriumsongaricumRupr.isaparasiticplantanditsmostcommonhostisNitrariaReceived24Augu
3、st2012tangutorumBobr.whichgrowsindesertareas.TosupportthesustainableuseandAccepted7December2012conservationofC.songaricum,geneticandphytochemicalstudieswereundertakenon16Availableonline12January2013differentpopulations.Sampleswereanalyzedusinginter-simpleseque
4、ncerepeat(ISSR)markersandhighperformanceliquidchromatography(HPLC)-fingerprinting.TotalKeywords:phenolicacidsweredeterminedbyUVspectrophotometry.Atthepopulationlevel,CynomoriumsongaricumRupr.percentageofpolymorphicbands(PPB)rangedfrom29.89to64.94%,Nei’sgeneticI
5、SSRdiversityindex(H)rangedfrom0.0931to0.2055,Shannon’sinformationindex(I)rangedHPLC-fingerprintingTotalphenolicacidsfrom0.1422to0.3140.16populationswereclassifiedintofourchemotypesaccordingtoHPLC-fingerprintinganalysis.Totalphenolicacidscontentsrangedfrom56.79to1
6、23.50mgg1.TheresultsofthegeneticandchemicalstudiesareusedtosuggestaplantingandconservationsystemforC.songaricum.Ó2012ElsevierLtd.Allrightsreserved.1.IntroductionCynomoriumsongaricumRupr.isaperennialparasiticherbwhichmainlyparasitesontherootofNitrariaL.(Huange
7、tal.,2010).ItsmostcommonhostisNitrariatangutorumBobr.(Dongetal.,2011)whichplaysanimportantecologicalroleasawindbreakandinsandfixation.InChina,C.songaricumdistributesprimarilyinnorthwestdesertandsalineareas.Itiswidelyusedforthetreatmentofdigestivedisorders,dyspe
8、psia,“kidney”deficiency,lumbagoanddiarrhea(Maetal.,2008).PolyphenolisakindofimportantcompoundsinC.songaricum.Thecompoundsincludetannins,(þ)-catechinandprocya-nidins(Kumazawaetal.,20
