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1、PlantScience170(2006)407412www.elsevier.com/locate/plantsciGeneticdiversityofpopuluscathayanaRehdpopulationsinsouthwesternchinarevealedbyISSRmarkersa,ba,ba,bcZhuoxuanLu,YuhuaWang,YouhongPeng,HelenaKorpelainen,ChunyangLia,*aChengduInstituteofBiology,ChineseAcademyofSciences,P.O.Box416,Chengdu6100
2、41,PRChinabGraduateSchooloftheChineseAcademyofSciences,Beijing100039,PRChinacDepartmentofAppliedBiology,P.O.Box27,FI-00014UniversityofHelsinki,FinlandReceived6July2005;receivedinrevisedform25September2005;accepted27September2005Availableonline11October2005AbstractAsanativespeciestoChina,Populusc
3、athayanaRehdiswell-adaptedtothewetandcoldenvironmentswhereitoccurs.ItisconsideredtobeanimportantreforestationspeciesinwesternChina.Inthepresentstudy,wesurveyedthelevelofgeneticvariationandthepatternofgeneticstructureinsevennaturalpopulationsofP.cathayana,originatingfromthesoutheasternQinghai-Tib
4、etanPlateauofChina,byusingISSR(inter-simplesequencerepeats)markers.Basedoneightprimers,158clearandreproducibleDNAfragmentsweregenerated,ofwhich156(98.7%)werepolymorphic.TheaveragevalueofNeisgenediversity(h)equaled0.331.Thecoefficientofgeneticdifferentiation(GST)equaled0.477,whichmeansthat47.7%oft
5、hetotalmolecularvarianceexistedamongpopulations.SuchahighlevelofdivergencepresentamongpopulationsmaybecausedbythecomplextopographyandvariableclimaticconditionspresentinthesoutheasternQinghai-TibetanPlateauwhicheffectivelyrestrictgeneflow.Moreover,thereisalackofsignificantassociationbetweengenetica
6、ndgeographicaldistances(r=0.3122,P>0.05)inthepopulationsofP.cathayana.Theapplicationofanovelmethod,whichcombinesgeographicalcoordinatesandgeneticdifferentiationtodetectbarriersforgeneflow,allowedustoidentifytwozonesofloweredgeneflow.TheISSRmethodusedinthepresentgeneticfingerprinting-basedstudyprove
7、dtobeaneffectivehigh-resolutiontoolforidentifyingpatternsofgeneticvariationpresentinforesttrees.InthecaseofP.cathayana,suchinformationisgreatlyneededforplanningconservationgeneticactionstoensurethemaintenanceofappropriatelev
