肝素钠抗凝血浆的心肌酶活力分析

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1、【摘要】目的分析肝素钠抗凝的血浆肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性，探讨肝素钠对心肌酶活力的影响。方法收集经体检证实健康个体113例，其中男性61例，女性52例，同时采集他们的肝素钠抗凝血浆和血清。通过连续监测法测定血浆和血清肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性。结果肝素钠抗凝血浆的肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性均值分别为95.4±50.3u/L、13.1±8.1u/L、184.0±41.9u/L、122.7±32.9u/

2、L、26.3±17.9u/L，而血清对照组的肌酸激酶、肌酸激酶同工酶MB、乳酸脱氢酶、α羟丁酸脱氢酶、天门冬氨酸氨基转移酶的活性均值分别为95.9±51.4u/L、12.7±7.5u/L、187.1±36.3u/L、119.6±29.2u/L、26.5±18.5u/L，两种标本的心肌酶活性无显著性差异（所有P值均大于0.05）。结论肝素钠对心肌酶活性无显著影响，肝素钠抗凝血浆可作为心肌酶活性测定的标本，可为临床抢救急性心肌梗死赢得宝贵时间。【关键词】心肌酶；酶活性；肝素钠；血浆ActivityAnalysisofPlasmaMyocardialEnzymeAntiCoagu

3、latedbyHeparinSodiumCAIChaomin,LIXiaoyun,PENGYunsheng,LIUQianru,MOBaomei,WANGXieheng.DepartmentofClinicalLaboratory,ThePeople’sHospitalofBaoan,Shenzhen,Shenzhen,518101,ChinaAbstract：ObjectiveThisstudyaimedtoanalysetheactivityofcreatinephospokinase,creatinephospokinaseisoenzymeMB,lactic

4、dehydrogenase,αhydroxybutyratedehydrogenase,andaspartateaminotransferaseinplasmaanticoagulatedbyheparinsodium,andtoexploretheeffectofheparinsodiumonmyocardialenzymeactivity.MethodsPeripheralbloodsampleswereobtainedfrom113healthyindividuals(61malesand52females).Plasmaanticoagulatedbyhepari

5、nsodiumandserumwereseparatedfromperipheralblood.Theenzymeactivitywasdeterminedbycontinuousmonitoringassayforcreatinephospokinase,creatinephospokinaseisoenzymeMB,lacticdehydrogenase,αhydroxybutyratedehydrogenase,andaspartateaminotransferase.ResultsTheenzymeactivityofplasmaanticoagulatedbyhe

6、parinsodiumwere95.4±50.3u/L,13.1±8.1u/L,184.0±41.9u/L,122.7±32.9u/L,and26.3±17.9u/Lforcreatinephospokinase,creatinephospokinaseisoenzymeMB,lacticdehydrogenase,αhydroxybutyratedehydrogenase,andaspartateaminotransferase,respectively.Whiletheenzymeactivityofserumwere95.9±51.4u/L,12.7±7.5u/L,18

7、7.1±36.3u/L,119.6±29.2u/L,and26.5±18.5u/Lforcreatinephospokinase,creatinephospokinaseisoenzymeMB,lacticdehydrogenase,αhydroxybutyratedehydrogenase,andaspartateaminotransferase,respectively.Therewasnosignificantdifferencebetweenplasmaanticoagulate