最新Expression of an antioxidative enzyme in the normal and 在正常和抗氧化酶的表达课件ppt.ppt

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1、Expressionofanantioxidativeenzymeinthenormaland在正常和抗氧化酶的表达SignalingNeuronWhydocellsdegenerate?1.FreeradicalsFreeradicalsarehighlyunstablechemicalsdamagecellstructures.Moststablecompoundsinthebodypossessapairofelectrons.Whenanelectronoftheelectronpairgetsstrippedawayi

2、tscalledafreeradical.Theyarehighlyunstablebecausetheseekothercompoundsandbreakbondsbetweenstablecompoundscausingachainreaction.Freeradicalscancomefromenvironmentalpollution,radiation,cigarettesmoke,chemicals,andherbicides.Whydocellsdegenerate?2.GeneticsTherearesevera

3、lgeneticmutationsandafewgenesthatarelinkedtoPD.Inheritingmutatedgenesmaymethesamegenesthatarealteredsporadicallybyenvironmentalfactorsandtoxins.alpha-synuclein-ThisgenewasmutatedinfamilieswithfamilialPDParkin-encodedintoaproteinwhichfunctionstohelpcellsbreakdownandrec

4、ycleproteinsDJ-1-normallyhelpscontrolgeneactivityandprotectcellsfromoxidativestressPINK1-whenitismutateditincreasesvulnerabilitytocellularstressLRRK2-linkedtolateonsetoffamilialPDandasmallpercentageinsporadicPDWhydocellsdegenerate?3.AgingInsomeindividuals,thenormal,a

5、ge-relatedwearingawayofdopamine-producingneuronsaccelerates.ThistheoryissupportedbythefactthatthelossofantioxidativeprotectivemechanismsisassociatedwithbothParkinson'sdiseaseandincreasingage.NAD(P)H:Quinoneoxidoreductase(NQO1)detoxicationenzymecatalysesthetwo-electron

6、reductionofDAQsintoDAhydroquinone,arelativelyredoxstableentitythatnotonlylacksmajorelectrophilicreactivitybutisalsoamenabletofurtherdetoxicationbyphaseIIenzymesRationaleexaminethecellularexpressionofNQO1inthebrainofalargeseriesofidiopathic(noknowncause)PDpatientsandag

7、e-matchedcontrolsdataonthecellularlocalizationofNQO1intheParkinsonianSNpcislackinginvestigatethepotentialroleofNQO1inthepathogenesisofPDSpecificityofNQO1immunocytochemistryImmunohistochemicalstainingwithantibodiesraisedagainstNQO1proteinshowsexpressionofNQO1innormalre

8、spiratoryepitheliumandNSCLC(adenocarcinoma).Noexpressioninsmallcelllungcancer(SCLC),surroundinglymphoidcellsorsupportingstro