微泡-脂质体复合物ppt课件.pptx

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1、Paclitaxelloadedliposomesdecoratedwithamultifunctionaltandempeptideforgliomatargetingcellpenetratingpeptides(CPP),aclassofdiverseshortpeptideswidelyusedforsiRNA,proteinsandsmallmoleculardrugsdelivery,alsohavetheabilitytocarrydrugstopenetrateBBBefficiently，non-specificaffinitytodifferentcells,Lea

2、dtounwantedtoxicitytonormalbraintissues.addaselectivedomaintoCPPorenhancethepenetratingcapabilityofspecificligands，，atandempeptidewithmultifunctionswasdesignedhere.（Thiskindoftandempeptidewasconsistedofaspecificpeptideinthefrontpositionandacellpenetratingpeptideinthebackendsothatitcouldpossessbo

3、thspecifictargetingandhighpenetratingability.）设计思路材料cyclicRGD[c(RGDfK)]andocta-arginine(R8)（以酰胺键连接，赖氨酸与精氨酸）Paclitaxel-liposome–microbubblecomplexesasultrasound-triggeredtherapeuticdrugdeliverycarriersLMC-DOX-us已经有人做过了，所以选择了紫杉醇？微泡原理将少量携带基因或药物的微泡经外周静脉或局部注射后，微泡流经靶向部位时进行超声检查，即得到超声影像。当超声影像图证明超声准确定位于

4、靶向部位时，增加声压使之达到一定强度，即可使微泡破裂产生空化效应，空化效应是指存在于液态物质中的微小空泡（空化核）在高强度超声的作用下被激发，空化核急剧膨胀和收缩直至爆裂，此过程中空化核吸收了大量的声能，并将能量集中释放在极小的区域，核内局部温度和压力急剧升高，随之产生强大冲击波、内切力、高速微束射流及自由基等二次效应，不仅使微血管破裂，血管内皮细胞收缩，细胞间隙增宽，微血管壁的通透性增大，还可使细胞膜的完整性遭到破坏，产生暂时性、可逆性的小孔，增加了细胞膜的通透性 ，均能够增强载药微泡对基因或药物的转移Reversalofmultidrugresistancephenotypeinh

5、umanbreastcancercellsusingdoxorubicin-liposome–microbubblecomplexesassistedbyultrasoundKeywordsDoxorubicin-liposome–microbubblecomplexes;Multidrugresistance;Ultrasound;Doxorubicin;Drugdelivery1,2distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethyleneglycol)-2000](DSPE-PEG2000)1,2-dis

6、tearoyl-sn-glycero-3-phosphatidylcholine(DSPC),dipalmitoylphosphatidylcholine（DPPC）1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethyleneGlycol)2000](DSPE-PEG2000-Biotin)Perfluoropropane(C3F8)材料DPPC:cholesterol:DSPE-PEG-biotininthemolarratioof60:40:5（薄膜-透析法）2.2.Preparationofbio

7、tinylatedDOX-liposomes(DL)2.3.PreparationofMBsandDOX-liposome–microbubblecomplexes(DLMC)2.4.CellcultureThedoxorubicinresistantMCF-7cellline2.5.DeterminationofDOXuptakeandretentioninDOX-resistantcells3.8.DownregulationofP-gly