磷酸钠缓冲液 配置比例.doc

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1、0.2MNa2HPO4：称取71.6gNa2HPO4-12H2O，溶于1000ml水0.2MNaH2PO4：称取31.2gNaH2PO4-2H2O，溶于1000ml水另：其它各种另PH值的0.2MPB（100ml)配方：pH    0.2MNaH2PO4（ml）      0.2MNa2HPO4（ml）5.7        93.5                  6.55.8        92                    8        5.9        90                    106.0        87.7     

2、             12.36.1        85                    156.2        81.5                  18.56.3        77.5                  22.56.4        73.5                  26.56.5        68.5                  31.56.6        62.5                  37.56.7        56.5                  43.56.8        

3、51                  496.9          45                  557.0          38                  627.1          33                  677.2        28                  727.3          23                  777.4          19ml                81ml7.5          16                  847.6          13   

4、               877.7        10.5                  90.57.8          8.5                  91.57.9        7                    938.0        5.3                  94.7