建立基因敲除小鼠颈动脉粥样硬化模型课件

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1、LipoproteinLipase(LPL)DeficiencyIncreasesPlasmaLipidLevelandEnhancesNeointimaFormationAfterCarotidArteryInjuryinapoEKnock-outMiceCandidategenesinlipoproteinmetabolismLusisetalCirculation.2004LipoproteinLipaseintheArterialWallPentikäinenetalArterioscler

2、ThrombVascBiol.2002LipoproteinLipaseintheArterialWallPentikäinenetalArteriosclerThrombVascBiol.2002HypothesisLPLArterialresponsetoinjuryhyperlipidemicconditions？A1x2-mmstripoffilterpapersaturatedwith10%FeCl3solutionwasappliedtotheadventitialsurfaceofth

3、esurgicallyexposedcarotidarteryfor3.0minutes,thenremoved.Thesurgicalincisionwassuturedwith4–0monofilament,andtheanimalwasreturnedtoitscage.CommoncarotidarteriesofapoE-/-,LPL+/-(ELK)mice(A)andapoE-/-,LPL+/+(EK)mice(B)8weeksafterferricchlorideinjuryneoin

4、timaformationaftercarotidarteryinjurybyFeCl3for8weeksELKcarotidarterystainedwithH&EEKnoninjuredinjuredneointimaformationaftercarotidarteryinjurybyFeCl3for8weekscarotidarterystainedwithH&EELKEKnoninjuredinjuredMeanIntimalandMedialCross-sectionalAreasofE

5、KandELKMice8WeeksafterInjuryEKELK***EKELKmedia2.95x104±20005.98x104±1000intimal4.01x104±60008.15x104±2000lipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcontrolcarotidarterystainedwithOROnoninjuredinjuredlipiddepositaftercarotidarteryinjurybyF

6、eCl3for8weeksELKEKcarotidarterystainedwithOROnoninjuredinjuredlipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcarotidarterycounterstainedwithOROandhaematoxylininjurednoninjuredlipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcarotidart

7、erycounterstainedwithOROandhaematoxylinnoninjuredinjuredMacrophagestainfor8weeksELKEKELKEKVonWillebrandFactorStainfor8weeks0week4weeks8weeksELK（n=4）61.52±11.09113.9±5.98270.17±24.29EK（n=3）53.96±18.1761.61±3.1485.07±9.40Pvalue0.760.00150.0016****NS0week

8、4weeks8weeksELK(n=4)395.5±34.89718.7±40.52939.32±46.67EK（n=3）239.6±59.77530.7±59.52686.08±56.66Pvalue0.0970.040.018NS**0week4weeks8weeksELK(n=4)118.3±7.9164.54±6.10109.7±4.46EK(n=3)85.19±17.7461.61±3.14109.53±2.90Pvalue0.190.680.980week