建立基因敲除小鼠颈动脉粥样硬化模型课件

《建立基因敲除小鼠颈动脉粥样硬化模型课件》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。

1、LipoproteinLipase(LPL)DeficiencyIncreasesPlasmaLipidLevelandEnhancesNeointimaFormationAfterCarotidArteryInjuryinapoEKnock-outMiceCandidategenesinlipoproteinmetabolismLusisetalCirculation.2004LipoproteinLipaseintheArterialWallPentikäinenetalArteriosclerThrombVascBiol.2002LipoproteinLi

2、paseintheArterialWallPentikäinenetalArteriosclerThrombVascBiol.2002HypothesisLPLArterialresponsetoinjuryhyperlipidemicconditions？A1x2-mmstripoffilterpapersaturatedwith10%FeCl3solutionwasappliedtotheadventitialsurfaceofthesurgicallyexposedcarotidarteryfor3.0minutes,thenremoved.Thesurg

3、icalincisionwassuturedwith4–0monofilament,andtheanimalwasreturnedtoitscage.CommoncarotidarteriesofapoE-/-,LPL+/-(ELK)mice(A)andapoE-/-,LPL+/+(EK)mice(B)8weeksafterferricchlorideinjuryneointimaformationaftercarotidarteryinjurybyFeCl3for8weeksELKcarotidarterystainedwithH&EEKnoninjuredi

4、njuredneointimaformationaftercarotidarteryinjurybyFeCl3for8weekscarotidarterystainedwithH&EELKEKnoninjuredinjuredMeanIntimalandMedialCross-sectionalAreasofEKandELKMice8WeeksafterInjuryEKELK***EKELKmedia2.95x104±20005.98x104±1000intimal4.01x104±60008.15x104±2000lipiddepositaftercaroti

5、darteryinjurybyFeCl3for8weeksELKEKcontrolcarotidarterystainedwithOROnoninjuredinjuredlipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcarotidarterystainedwithOROnoninjuredinjuredlipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcarotidarterycounterstainedwithOROandhaem

6、atoxylininjurednoninjuredlipiddepositaftercarotidarteryinjurybyFeCl3for8weeksELKEKcarotidarterycounterstainedwithOROandhaematoxylinnoninjuredinjuredMacrophagestainfor8weeksELKEKELKEKVonWillebrandFactorStainfor8weeks0week4weeks8weeksELK（n=4）61.52±11.09113.9±5.98270.17±24.29EK（n=3）53.9

7、6±18.1761.61±3.1485.07±9.40Pvalue0.760.00150.0016****NS0week4weeks8weeksELK(n=4)395.5±34.89718.7±40.52939.32±46.67EK（n=3）239.6±59.77530.7±59.52686.08±56.66Pvalue0.0970.040.018NS**0week4weeks8weeksELK(n=4)118.3±7.9164.54±6.10109.7±4.46EK(n=3)85.19±17.7461.61±3.14109.53±2.90Pvalue0.190

8、.680.980week