通窍活血汤改善拟血管性痴呆大鼠学习记忆作用的研究.pdf

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1、通窍活血汤改善拟血管性痴呆大鼠学习记忆作用的研究葛朝亮，汪宁。(1．安徽医科大学第一附属医院药剂科，合肥230022；2．安徽中医学院，安徽省中药研究与开发重点实验室，合肥230038)摘要：目的研究通窍活血汤对拟血管性痴呆(vasculardementia，VD)大鼠学习记忆作用及脑皮质中乙酰胆碱含量的影响，探讨通窍活血汤对拟血管性痴呆大鼠的作用机制。方法采用颈总动脉(CCA)注射混合血栓诱导剂法制作拟血管性痴呆大鼠模型。通过八臂迷宫检测大鼠工作记忆错误次数、参考记忆错误；光镜(HE染色)观察拟血管性痴呆大鼠脑组织海马CA1区病理形态的改变；酶联免疫吸附试验(ELISA)方法

2、检测拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量。结果通窍活血汤高、中剂量能显著减少拟血管性痴呆大鼠的工作记忆错误次数、参考记忆错误次数(P<0．O1)；明显改善拟血管性痴呆大鼠脑组织海马CA1区病理形态学异常；显著提高拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量(P<0．01)。结论通窍活血汤能明显改善拟血管性痴呆大鼠的学习、记忆能力。其作用机制可能与改善拟血管性痴呆大鼠脑组织海马CA1区的椎体细胞的病理形态的异常，提高拟血管性痴呆大鼠脑皮质中乙酰胆碱的含量有关。关键词：通窍活血汤；血管性痴呆；海马CA1区；乙酰胆碱中图分类号：R965文献标志码：A文章编号：1001—2494(2012

3、)08—0590—05ActivityofTongqiaoHuoxueDecoctionontheLearningandMemoryAbilityofRatswithVascularDemen—tiainRatsGEChao．1iang一，WANGNing2(1．TheFirstAffiliatedHospitalofAnhuiMedicalUniversity，Hefei230022，China；2．AnhuiTra—ditionalChineseMedicineCollege，AahtdProvinceKeyLaboratoryofChineseMedicineResear

4、chandDevelopment，Hefei230038，China)ABSTRACT：OBJECTIVETostudytheactivityofTQHXDonthelearningandmemoryabilityofratswithvasculardementia(VD)anditseffectsonthecontentofAchincerebralcortex．AndtoinvestigatetheactionmechanismofTQHXDonVDinrats．METH—oDSVDmodelwasmadebycommoncarotidarteryinjectionofac

5、o—thrombusinducer．The8-armradialmazeexperimentwasadopt—edtoevaluatethetimesofworkingmemoryerrorsandreferencememoryelrors．ThechangesofthepathologicalareainhippocampusCA1wereobservedbyopticalmicroscope．Enzyme—linkedimmunosorbentassaywasusedtodeterminetheconcentrationofAchinratcerebralcortex．RE

6、SULTSHighandmiddledoseofTQXHDsignificantlyreducethetimesofworkingmemoryerrorsandreferencememoryerrors(P<0．01)，definitelyimprovedtheanormaliesofpathologicalareainhippocampalCA1，andsignificantlyincreasedthecontentofAchincerebralcortex(P<0．O1)．CONCLUSIONTQHXDcansignificantlyamelioratethelearnin

7、gandmemorya—bilityofinVDrats．ThemechanismmayberelatedtotheimprovementofthevertebralbodycellsanomaliesinthehippocampalCA1regionandincreasingthecontentoftheAchincerebralcortex．KEYWORDS：tongqiaohuoxuedecoction；vasculardementia；hippocampalCA1region；ace