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ID:5826964
大小:1.05 MB
页数:22页
时间:2017-12-13
《呼吸运动调节因素实验》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、实验五Theexperimentaboutregulatoryfactorsonrespiratorymovement呼吸运动调节因素实验Rhythmicbreathingexerciseundernormalconditionsandadaptabilityofrespiratorymovementinavarietyofphysiologicalconditionchangesdependsontheregulationoftheneuralmechanism.正常情况下的节律性呼吸运动以及在各种生理状态下呼吸运动所发生的适应性改变都有赖于神经机制的调节作用.Experimentalpr
2、inciplesThemedullaoblongataisthebasiccenterofregulatingrhythmicalrespiration.延髓是调节节律性呼吸的基本中枢。Reflexactivitieswhicharecausedbythestimulusfromrespiratorytract,chemicalchangesinlungsandblood,aretheimportantguaranteeforthenormalrhythmicbreathing.来自呼吸道、肺部及血液中化学成分改变的刺激所引起的反射活动,是实现正常节律性呼吸的重要保证。Experimenta
3、lprinciplesExperimentalobjective●Learningmethodsofrecordingtherespiratorymovement学习呼吸运动的记录方法●Toobservetheeffectofmorphineonrespirationandlearnhowtorescue观察吗啡对呼吸的影响及学习如何解救。Experimentalobjective●Toobservetheeffectsofnerve,bodyfluidfactorsonrespiratorymovement观察神经、体液对呼吸运动的影响Toverifytheregulationmechan
4、ismofrespiratorymovement验证呼吸运动的调节机制Totrainyourskillofhandlingchartpaper.训练处理图纸的能力ExperimentalequipmentsBL-420E+biologicalsignalcollectingandprocessingsystem(BL-420E+生物机能实验系统)endotrachealtube(气管插管)、respirationtransducer(呼吸换能器)、injectors(注射器20ml、5ml)surgicalinstruments(手术器械)、50cmrubbertube(胶管)Experim
5、entalanimalExperimentaldrugs20%urethane(乌拉坦/氨基甲酸乙酯)、1%morphine吗啡5%nikethamide尼可刹米naloxone纳洛酮3%lacticacid乳酸溶液、CO2airpocket气囊、N2airpocket气囊Ⅰanesthesiaandfix麻醉固定1.Weigh称重Experimentalprocedure实验步骤2.Injecturathane(5ml/kg,ivandip.)耳源静脉、腹腔注射20%乌拉坦慢3.fixtherabbit固定家兔(※maketheneckflat颈部平坦)Experimentalproced
6、ure1.Shearingoftheneck颈部剪毛2.Cutthecervicalskin4~5cmlongalongthemidline沿颈正中剪开皮肤3.Separatebluntlythesubcutaneoustissueandmuscletoexposethetrachea,andputalineunderthetrachea钝性分离皮下组织及肌肉,暴露气管,穿一条线备用。4.Findcarotidsheathonbothsidesoftrachea,thenseparatethebilateralvagalN(thethickest)andputlinesunderthem在气
7、管两侧找到颈动脉鞘,并分离双侧迷走N(最粗的),穿二条线备用。Ⅱ、operationsoftheneck颈部手术Ⅲ、trachealintubation气管插管1/31/2气管插管图示2cmthyroidcartilage甲状软骨于甲状软骨下方2cm处的气管软骨之间切开l/3~1/2气管圆周,并向上开倒T字型切口。用干棉球将气管内血液及分泌物擦干净,向肺方向插入气管插管,并结扎。ⅣConnecttheexpe
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