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ID:56804605
大小:718.50 KB
页数:25页
时间:2020-06-28
《大规模基因组测序中的信息分析.ppt》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、大规模基因组测序中的信息分析--拼接与注释大规模测序是基因组研究的最基本任务,它的每一个环节都与信息分析紧密相关。从测序仪的光密度采样与分析、碱基读出、载体标识与去除、拼接与组装、填补序列间隙、到重复序列标识、读框预测和基因标注的每一步都是紧密依赖基因组信息学的软件和数据库的。SequenceDataDistributionVectorSequencingandDataProcessingProcedureBasecallingRepeatMarkORFPredictionGeneAnnotationFinishingAssembleVectorMarkPhredPhd2fastaCr
2、ossmatchPhrapConsedRepeatmaskerGlimmerBlastxBlastnClastaltRNAscanQualityControlQualCalQualDrawQualStatCOGsSwiss-port,PIR,GDB,GenBankSequencingHowtofindthecodingregionsinrudeDNAsequence?StatisticalmethodandSequenceAlignmentMethodenevenpositionalbasefrequence(Dvalue)Neuralnetworkpredictingthespli
3、cingsitesFractaldimensionofexonsandintronsComplexityanalysisHowmanydifferentpatternsarethereintheareaofthedifferentDNAsequence?MethodandTechniquesinCryptologyCoincidentIndexsUnicityDistance#Hidden-Markovmethod(HMM)Glimmerv------NeuralnetworkmethodAimprovedback-propagation(BP)learningalgorithmhav
4、ebeenbuiltinthestudy.Onegoalofthisnetworkistofindthesplicingsitebetweenintronandexon.NeuralNetworkProcedurexl-1yW=synapticweightvectorofaneuroninlayerl=thresholdofaneuroninlayerlV=vectorofnetinternalactivitylevelsofneuronsY=vectoroffunctionsignalsofneuronsinlayerl(l)(l)(l)(l)ForwardComputationi
5、nputvectorxdesiredresponsevextordThenetinternalactivitylevelforneuronjinlayerlisIfneuronjisintheoutputlayer(i.e.,l=L),setHence,computetheerrorsignalBackwardComputationvectoroflocalgradientsofneuronsinlayerle=errorvectorrespresentedbye,e,….,easelements12qforneuronjinoutputlayerLforneuronjinh
6、iddenlayerlIteration-------------GU-----------------AG-------------splicingsitesexonexonintronpseudosplicingsites---------------GU------------------AG--------------successrate=38/60~64%FractaldimensionofexonandintronWeintroducethemappingfromnucleotidesequencestotwo-dimensionalmetricspacebyass
7、igningeachnucleotideafixedvectorandconstructingarepresentationbyjoiningthesevectorsend-to-end.FractalDimensionofExonandIntronSequencesDisthefractaldimensionandthereexistsself-similarityinthisrangeofthenucleotidesequence.----
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