蛋白质Geneontology,KEGG分析软件David使用方法介绍.ppt

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1、DAVIDDatabaseforAnnotation,VisualizationandIntegratedDiscoveryDAVID providesacomprehensivesetoffunctionalannotationtoolsforinvestigatorstounderstandbiologicalmeaningbehindlargelistofgenesIdentifyenrichedbiologicalthemes,particularlyGOtermsDiscoverenrichedfunctional-

2、relatedgenegroupsClusterredundantannotationtermsVisualizegenesonBioCarta&KEGGpathwaymapsDisplayrelatedmany-genes-to-many-termson2-Dview.SearchforotherfunctionallyrelatedgenesnotinthelistListinteractingproteinsExploregenenamesinbatchLinkgene-diseaseassociationsHighli

3、ghtproteinfunctionaldomainsandmotifsRedirecttorelatedliteraturesConvertgeneidentifiersfromonetypetoanother.GeneListManagerDAVIDAnalyticModulesAnygivengeneisassociatingwithasetofannotationterms.Ifgenessharesimilarsetofthoseterms,theyaremostlikelyinvolvedinsimilarbiol

4、ogicalmechanisms.Thealgorithmadoptskappastatisticstoquantitativelymeasurethedegreeoftheagreementhowgenessharethesimilarannotationterms.Kapparesultrangesfrom0to1.ThehigherthevalueofKappa,thestrongertheagreement.Kappamorethan0.7typicallyindicatesthatagreementoftwogene

5、sarestrong.Kappavaluesgreaterthan0.9areconsideredexcellent.ParameterPanelGeneClustersIdentifiedbyDAVIDUser’sgeneIDs&Names12Viewandselectannotationcategoriesofyourinterests333Percentage,e.g.44/163GenesfromthelistinvolvedinthisannotationcategorySinglechartreportonlyfo

6、rthisAnnotationcategoriesTablereportforallSelectedannotationcategoriesLinearorredundantchartreportofannotationtermsforallselectedannotationcategoriesaboveClusteredornon-redundantchartreportofannotationtermsforallselectedannotationcategoriesaboveDAVIDGeneIDConversion

7、Tool(DGCT)Ifasignificantportion(>20%)ofinputgeneIDsfailtobemappedtoaninternalDAVIDID,aspeciallydesignedmodule,theDAVIDGeneIDConversionTool,willstartuptohelpmapsuchIDs.DAVIDGeneNameBatchViewerThegenenamebatchviewerisabletoquicklyattachmeaningtoalistofgeneIDsbyrapidly

8、translatingthemintotheircorrespondinggenenames.beforeproceedingtoanalysiswithothermorecomprehensiveanalytictools,investigatorscanquicklygl