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时间:2020-06-04
《淮南麻黄鸡CRBP4基因多态性与早期产蛋性状和蛋品质的相关性.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、畜牧兽医学报2013,44(2):197—203ActaVeterinariaetZootechnicaSinica淮南麻黄鸡CRBP4基因多态性与早期产蛋性状和蛋品质的相关性李俊营,詹凯,刘伟,韩涛,李绍全。,柏如东。(1.安徽省农业科学院畜牧兽医研究所,合肥230031;2.安徽皖西麻黄鸡禽业有限公司,六安2374003.安徽农业大学生命科学学院,合肥230036)摘要:旨在探索CRBP4基因与淮南麻黄鸡产蛋性状和蛋品质的相关性,寻找可作为淮南麻黄鸡产蛋性状的重要分子标记。本研究利用PCR—SSCP技术和DNA测序技术对308只淮南麻黄鸡CRBP4基因遗传多态性
2、进行检测及基因型分析,并对CRBP4基因的多态位点与早期产蛋性状和3O周龄蛋品质进行相关分析。结果表明,在CRBP4基因exon2和exon3上存在3个突变位点,即C826T、C1240T和A1241G,其中C826T和A1241G导致了49Ser--~49Leu和97Ile一97Val突变,这3个突变位点都属于中度多态,基因型分布都处于Hardy-Weinberg平衡状态(P>O.05)。C826T位点与母鸡的开产蛋重呈显著相关,CC基因型有利于提高开产蛋重;构建的单倍型对开产蛋重的影响达到显著水平(P3、淮南麻黄鸡开产蛋重存在相关性。关键词:CRBP4基因;SNPs;早期产蛋性状;蛋品质中图分类号:$813.2文献标志码:A文章编号:0366—6964(2013)02—0197—07AssociationofPolymorphismsoftheCRBP4GenewithEarlyEggProductionTraitsandEggQualityofHuainanPartridgeChickenLIJun-ying,ZHANKai¨,LIUWei,HANTao,LIShao-quan,BAIRu-dong。(1.InstituteofAnimalHusbandryand4、VeterinaryMedicine,AnhuiAcademyofAgriculturalSciences,Hefei230031,China;2.AnhuiWanxiMahuangjiPoultryCo.,Ltd,Liu’an237400,China;3.CollegeofLifeScience,AnhuiAgriculturalUniversity,Hefei230036,China)Abstract:ThepurposeofthisstudywastoexploretheassociationsofpolymorphismsoftheCRBP4genewith5、egglayingtraitsandeggqualityandtofindimportantmarkersforthesetraitsofHuainanpartridgechicken.ThepolymorphismsofCRBP4geneweredetectedbyPCR—SSCPanddirectlysequencingin308Huainanpartridgechickenundercagecondition.TheassociationbetweenmutationsitesofCRBP4geneandearlyegglayingtraitsandeggqu6、alitywasanalyzed.TheresuItsshowedthat,therewerethreemutations,C826Tintheexon2,C124OTandAl~41Gintheexon3,respectively,andC826TandA1241Gresultedin49Ser---~49Leuand97Ile_’97Val,respectively.Thethreesitesbelongedtomoderatepolymorphism.Atthethreesites,thepopulationwasinHardy—Weinbergequilib7、rium(P>O.05).ThemutationsiteofC826Twassig-nificantlyassociatedwiththeeggweightatfirstlaying(P<0.05).TheCCgenotypewaspositivefortheeggweightatfirstlaying.ThehaplotypecombinationsconsistingofC826T,C1240TandA1241Gwassignificantlyassociatedwitheggweightatfirstlaying(P<0.05).Theresultsind
3、淮南麻黄鸡开产蛋重存在相关性。关键词:CRBP4基因;SNPs;早期产蛋性状;蛋品质中图分类号:$813.2文献标志码:A文章编号:0366—6964(2013)02—0197—07AssociationofPolymorphismsoftheCRBP4GenewithEarlyEggProductionTraitsandEggQualityofHuainanPartridgeChickenLIJun-ying,ZHANKai¨,LIUWei,HANTao,LIShao-quan,BAIRu-dong。(1.InstituteofAnimalHusbandryand
4、VeterinaryMedicine,AnhuiAcademyofAgriculturalSciences,Hefei230031,China;2.AnhuiWanxiMahuangjiPoultryCo.,Ltd,Liu’an237400,China;3.CollegeofLifeScience,AnhuiAgriculturalUniversity,Hefei230036,China)Abstract:ThepurposeofthisstudywastoexploretheassociationsofpolymorphismsoftheCRBP4genewith
5、egglayingtraitsandeggqualityandtofindimportantmarkersforthesetraitsofHuainanpartridgechicken.ThepolymorphismsofCRBP4geneweredetectedbyPCR—SSCPanddirectlysequencingin308Huainanpartridgechickenundercagecondition.TheassociationbetweenmutationsitesofCRBP4geneandearlyegglayingtraitsandeggqu
6、alitywasanalyzed.TheresuItsshowedthat,therewerethreemutations,C826Tintheexon2,C124OTandAl~41Gintheexon3,respectively,andC826TandA1241Gresultedin49Ser---~49Leuand97Ile_’97Val,respectively.Thethreesitesbelongedtomoderatepolymorphism.Atthethreesites,thepopulationwasinHardy—Weinbergequilib
7、rium(P>O.05).ThemutationsiteofC826Twassig-nificantlyassociatedwiththeeggweightatfirstlaying(P<0.05).TheCCgenotypewaspositivefortheeggweightatfirstlaying.ThehaplotypecombinationsconsistingofC826T,C1240TandA1241Gwassignificantlyassociatedwitheggweightatfirstlaying(P<0.05).Theresultsind
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