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《桑叶提取物1-DNJ对甜菜夜蛾幼虫生长和保护酶活性的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中圈摩学c墨攉2014,30(22):316—320ChineseAgriculturalScienceBulletin桑叶提取物1-DNJ对甜菜夜蛾幼虫生长和保护酶活性的影响张秀丽。,王炎,徐东2刘斌,张会慧。,敖红。,孙广玉(东北林业大学生命科学学院,哈尔滨150040;吉林吉农高新技术发展股份有限公司,吉林公主岭136100;安徽隆平高科种业有限公司,吉林公主岭136100)摘要:为研究桑树活性物质1.DNJ对甜菜夜蛾幼虫生长和体内保护酶活性的影响,将被不同浓度1-DNJ浸泡30S后的甘蓝小叶饲喂4龄的甜菜夜蛾幼虫。结果表明:甜菜夜蛾4龄幼虫对1
2、.DNJ在24、48h的拒食率分别为77.20%、92.01%,拒食中浓度AFC5。分别为0.48、0.21g/L;生长抑制检测表明,1-DNJ对于甜菜夜蛾生长的抑制率最高达到92.34%,说明1-NDJ对甜菜夜蛾的生长具有明显抑制作用;1-DNJ对甜菜夜蛾体内保护酶活性具有明显的影响,其中SOD和CAT活性,在各时段的检测结果均呈现先被诱导后被抑制,CAT活性较对照下降幅度明显,与对照组差异达到显著水平3、蛾幼虫体内保护酶系统对桑树活性物质1-DNJ反应敏感。本研究结果说明:桑树活性物质1-DNJ对甜菜夜蛾的生长和保护酶活性具有一定的影响,其结果可为将桑树作为开发植物源杀虫剂的原料提供基础资料和理论依据。关键词:桑树;次生代谢产物;1-DNJ;甜菜夜蛾;保护酶活性中图分类号:$481文献标志码:A论文编号:2014.0413Effectofl—DNJfromMulberryLeavesontheGrowthandProtectiveEnzymesofSpodopteraexiguaZhangXiuli,WangYan,XuDong2,LiuBin,Zha4、ngHuihui,AoHong,SunGuangyu。(CollegeoftifeScience,Noahe~tForestUniversity,Harbin150040;LongpingHigh—techSeedInd~tryCo.,Ltd.Jilin,GongzhulingJilin136100;3JinongHi一.Co.,Ltd.Jilin,GongzhulingJilin136100)Abstract:Inordertostudytheinfluenceofmulberryactivesubstancer1一NDJ)onthegrowthan5、denzymaticactivityof4s.exigua.whichwerefeedcabbageleavessoakedbydiferentconcentrationsfor30second.Theresultsshowedthatthehighestanti—feedingrateswere77.20%and92.01%,respectivelyat24hand48h,andtheAFC50were0.48and0.21L,respectively.Thedevelopmentalinhibitionratesdecreasedobviously6、withtheincreasing1一DNJconcentration,whichcouldupto92.34%.While,activityofsuperoxidedismutaseandcarboxylesteraseofthe4s.exiguawereinducedandtheninhibitedinaftertreatedwith1-DNJ.activityofcarboxylesterasedecreasedsignificantlythanCK<0.05),activityofperoxidasewerefewchangedby1一DNJ.7、Theactiontimeofanti—feedingandgrowthinhibitorywereloggedineffectsofprotectiveenzymesystem。suggestingthatprotectiveenzymesystemwassensitiveto1一DNJ.Theseresultsimpliedthatmulberrywastreasuriesofbioactivesubstancesusefulaspesticides,whichcouldprovidebasicdataandtheoretical基金项目:中央高校8、基本科研业务费专项资金项目“桑叶浸提液对菜青虫生长的干扰机理及活性成分的分离”(DL11BA1
3、蛾幼虫体内保护酶系统对桑树活性物质1-DNJ反应敏感。本研究结果说明:桑树活性物质1-DNJ对甜菜夜蛾的生长和保护酶活性具有一定的影响,其结果可为将桑树作为开发植物源杀虫剂的原料提供基础资料和理论依据。关键词:桑树;次生代谢产物;1-DNJ;甜菜夜蛾;保护酶活性中图分类号:$481文献标志码:A论文编号:2014.0413Effectofl—DNJfromMulberryLeavesontheGrowthandProtectiveEnzymesofSpodopteraexiguaZhangXiuli,WangYan,XuDong2,LiuBin,Zha
4、ngHuihui,AoHong,SunGuangyu。(CollegeoftifeScience,Noahe~tForestUniversity,Harbin150040;LongpingHigh—techSeedInd~tryCo.,Ltd.Jilin,GongzhulingJilin136100;3JinongHi一.Co.,Ltd.Jilin,GongzhulingJilin136100)Abstract:Inordertostudytheinfluenceofmulberryactivesubstancer1一NDJ)onthegrowthan
5、denzymaticactivityof4s.exigua.whichwerefeedcabbageleavessoakedbydiferentconcentrationsfor30second.Theresultsshowedthatthehighestanti—feedingrateswere77.20%and92.01%,respectivelyat24hand48h,andtheAFC50were0.48and0.21L,respectively.Thedevelopmentalinhibitionratesdecreasedobviously
6、withtheincreasing1一DNJconcentration,whichcouldupto92.34%.While,activityofsuperoxidedismutaseandcarboxylesteraseofthe4s.exiguawereinducedandtheninhibitedinaftertreatedwith1-DNJ.activityofcarboxylesterasedecreasedsignificantlythanCK<0.05),activityofperoxidasewerefewchangedby1一DNJ.
7、Theactiontimeofanti—feedingandgrowthinhibitorywereloggedineffectsofprotectiveenzymesystem。suggestingthatprotectiveenzymesystemwassensitiveto1一DNJ.Theseresultsimpliedthatmulberrywastreasuriesofbioactivesubstancesusefulaspesticides,whichcouldprovidebasicdataandtheoretical基金项目:中央高校
8、基本科研业务费专项资金项目“桑叶浸提液对菜青虫生长的干扰机理及活性成分的分离”(DL11BA1
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