VEGFA,HIF1A和EPAS1基因多态性与猪繁殖性状的相关研究.pdf

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1、第32卷第3期上海交通大学学报(农业科学版)Vo1．32No．32014年6月JOURNALOFSHANGHAIJIAOTONGUNIVERSITY(AGRICULTURALSCIENCE)Jun．2014文章编号：1671—9964(2014)03—0007—08D0I：10．3969／J．ISSN．1671—9964．2014．03．002PolymorphismsoftheSwineVEGFA，HIF1AandEPAS1GenesandTheirCorrelationwithReproduct

2、iveTraitsZHANGXiang—zhe。，YANGLun。，CHENQiang，TANGui一ng，CHENJian，YANGuo—xiang，PANYu—chun1,3(1．SchoolofAgricultureandBiology，ShanghaiJiaotongUniversity，Shanghai200240，China；2．Bio-XCenter，ShanghaiJiaotongUniversity，Shanghai200030，China；3．ShanghaiKeyLabofVe

3、terinaryBiotechnology，Shanghai200240，China；4．ShanghaiXiangxinLivestockPoultryCo．Ltd．，Shanghai201302，China)Abstract：TheaimofthisworkwastotrytodiscoverpolymorphismsandstudytheeffectsoftheswinegenesVElGFA，HF1AandEPAS1onreproductivetraits．Afteralignmentofs

4、equencesofdifferentindividuals，27potentialSNPsweredetected．Four(g．1438C~G，g．1811C>G，g．6973G~Tandg．12532C>T)oftheseSNPscanbedetectedbyPCR_RFLPusingdigestionofthePCRfragmentwithB5H2工，PspEI，Eco32IandBcn工intheintronintheporcineVEGFAgene．TheotherSNPscannotb

5、erecognizedbyPCR—RFLP．TheallelesoffourSNPsweredistributedwithobviousdifferenceinallelefrequencybetweenMeishanpigandFrenchLargeWhitepig．Theg．6973G>TpolymorphismoftheVEGFAgeneshowedsignificantcorrelationwithTNBandNBAinlaterparities(P

6、en、／=GFc4haplotypes(GTCT，CGCC，GTGC，CGCT，CGCC，GGGC，GGCT，GGGT，GTCCandCTGT)weredetectedinMeishanpigs，whileonlytwohaplotypes(GTGCandGTCT)inFrenchLargeWhitepigs．TheresultsshowedthatGGGChaplotypehadignificanteffectsonTNB／NBAinsecondandsubsequentlitters(P<0．0

7、5)．GTGChaplotypehadasignificanteffectOnTNBinfirstlitter(P

8、atedwithswinereproductivetraitsandfurtherinvestigationwillbenecessarytoillustratetheunderlyingmechanism．Keywords：swine；ⅦGFA；polymorphism；reproductivetraitsGFA，HF1A和EPAS1基因多态性与猪繁殖性状的相关研究张向结一，杨仑，陈强，谭桂芳，陈剑，严国祥，潘玉春。(1．上海交通大学农业与生物学院，上海200240