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《淫羊藿苷延长秀丽线虫寿命与机制.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、老年医学与保健2013年第l9卷第6期GeriatrHealthCare,2013,Vo1.19.No.6●论著●淫羊藿苷延长秀丽线虫寿命与机制吴俊珍夏世金孙涛蔡外娇黄建华宁友吴金峰彭正羽【摘要】目的探讨淫羊藿苷(Icariin,Ica)对秀丽线虫寿命的干预效果与机制,为筛选延缓衰老、延长寿命的药物提供实验依据。方法将秀丽线虫分为空白对照组、Ica5mg/L、10mg/L、15mg/L、2Omg/L各剂量干预实验组,观察在Ica不同梯度剂量作用下秀丽线虫寿命的变化,以明确Ica作用的最佳剂量。接着设立秀丽线虫空白对照组和
2、Ica(15mg/L)干预组,评价Ica对秀丽线虫平均寿命、生殖能力的干预效果;同时进行急性热应激和急性氧化应激实验,观察应激时线虫的平均生存时间,探讨Ica延长线虫寿命的机制。结果15mg/L的Ica是延长秀丽线虫寿命的最佳剂量。Ica组线虫的平均寿命明显长于空白对照组【(31.87±4.46)dvs.(20.13土2.81)d】(P<0.O1);Ica组线虫生殖高峰期子代数目比空白对照组显著增加(151+21.13v8.122±17.09,P3、明显长于空白对照组【(9.53±1.34)hvs.(5.97+0.84)h,(8.34士1.17)hvs.(5.77士O.81)hi(P<0.01o结论淫羊藿苷能延长秀丽线虫的寿命,其机制可能与淫羊藿苷提高线虫的应激能力有关。[关键词】寿命;衰老;秀丽线虫;淫羊藿苷;热应激;氧化应激;生殖能力【中图分类号】R339.8[文献标识码】A[文章编号】1008—8296(2013)一06—349—05D0I:10.3969~.issn.1008—8296.2013—06EffectoficariinoillifespanofC4、aenorhabditiseleganswUJun—zhen.X1AShi-jin*,SUNTao.CAIWai-jiao.HUANGJian—hua,N1NGYou.wJin-feng,PENGZheng-yu.DepartmentofGeriatrics,HuadongHospital,FudanUniversity,Shanghai200040,ChinaCorrespondingauthor:XIAShi-jin,E—mail:xiashijinhd@163.comIAbstract】ObjectiveToeva5、luatetheeffectofIcariin(Ica)onlifespanofCelegansinordertoscreendrugsforlongevity.Methods(1)Dosage—effecttest:eelegans,whichweretreatedwithIca0mg/L,Ica5mg/L,10mg/L,15mg/L,2Omg/L,respectively,weretestedbylifespanassaytodeterminethebesteffectivedosagesofIca.(2)Lifes6、panassay:C.elegansweredividedintocontrolgroupandIca(15mg/L)treatmentgroup.Lifespanassaywasusedtoevaluatethelife-extensioneffectofIcaonCelegans.(3)Fecunditytest:eelegansfecundityintheIca(15mg/L)treatmentandcontrolgroupswasassessedbyevaluatingtheeffectofIcaonthewor7、ms’reproduction.(4)Stressresistancetest:thelifespanofeelegansintheIca(15mg/L)treatmentandcontrolgroupswereassessedbyacuteheatandoxidativestresstreatmenttoexploretheinitialmechanismsinvolvedintheeffectofIcaonlifespan.Results15mg/LofIcawasthebestdosagetomadeeelegan8、sgetthemaximumlifespan.TheaveragelifespanofC.elegansintheIca·treatedgroupwassig—nificantlylongerthanthecontrolgroup【(31.87±4.46)dvs.(20.13±2.81)d】(P
3、明显长于空白对照组【(9.53±1.34)hvs.(5.97+0.84)h,(8.34士1.17)hvs.(5.77士O.81)hi(P<0.01o结论淫羊藿苷能延长秀丽线虫的寿命,其机制可能与淫羊藿苷提高线虫的应激能力有关。[关键词】寿命;衰老;秀丽线虫;淫羊藿苷;热应激;氧化应激;生殖能力【中图分类号】R339.8[文献标识码】A[文章编号】1008—8296(2013)一06—349—05D0I:10.3969~.issn.1008—8296.2013—06EffectoficariinoillifespanofC
4、aenorhabditiseleganswUJun—zhen.X1AShi-jin*,SUNTao.CAIWai-jiao.HUANGJian—hua,N1NGYou.wJin-feng,PENGZheng-yu.DepartmentofGeriatrics,HuadongHospital,FudanUniversity,Shanghai200040,ChinaCorrespondingauthor:XIAShi-jin,E—mail:xiashijinhd@163.comIAbstract】ObjectiveToeva
5、luatetheeffectofIcariin(Ica)onlifespanofCelegansinordertoscreendrugsforlongevity.Methods(1)Dosage—effecttest:eelegans,whichweretreatedwithIca0mg/L,Ica5mg/L,10mg/L,15mg/L,2Omg/L,respectively,weretestedbylifespanassaytodeterminethebesteffectivedosagesofIca.(2)Lifes
6、panassay:C.elegansweredividedintocontrolgroupandIca(15mg/L)treatmentgroup.Lifespanassaywasusedtoevaluatethelife-extensioneffectofIcaonCelegans.(3)Fecunditytest:eelegansfecundityintheIca(15mg/L)treatmentandcontrolgroupswasassessedbyevaluatingtheeffectofIcaonthewor
7、ms’reproduction.(4)Stressresistancetest:thelifespanofeelegansintheIca(15mg/L)treatmentandcontrolgroupswereassessedbyacuteheatandoxidativestresstreatmenttoexploretheinitialmechanismsinvolvedintheeffectofIcaonlifespan.Results15mg/LofIcawasthebestdosagetomadeeelegan
8、sgetthemaximumlifespan.TheaveragelifespanofC.elegansintheIca·treatedgroupwassig—nificantlylongerthanthecontrolgroup【(31.87±4.46)dvs.(20.13±2.81)d】(P
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