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1、摘要本试验选用市农信油菜种子和省盛鸿油菜种子为材料,在实验室采用培养皿滤纸发芽法[1],在不同浓度的NaCl单盐胁迫下进行种子发芽实验(浓度梯度设为0、0.2%、0.4%、0.6%、0.8%、1.0%、1.2%七个梯度),测定发芽率、发芽指数、发芽势和活力指数等指标。结果表明:在单盐胁迫下,随着胁迫浓度的增加,油菜种子的发芽率、发芽指数、发芽势和活力指数均有不同程度的降低,相对盐害率升高,种子开始发芽的时间推迟且发芽过程延长;NaCl的胁迫对油菜种子萌发的抑制作用很大,其中浓度0.4%NaCl的胁迫
2、对油菜发芽率的抑制均达到了50%以上分别为88%、88%,在浓度为2.0%的NaCl胁迫下两种供试材料的发芽率均为零;盐胁迫对种子发芽有抑制作用,但低浓度盐胁迫对种子发芽有促进作用。农信油菜种子优于盛鸿油菜种子的抗盐性。关键词:氯化钠胁迫;油菜;种子萌发AbstractThisexperimentchoosesitsrapeseedandHarbininHeilongjiangShengHongrapeseedinthelaboratoryformaterial,usingflasksfilterm
3、ethod[1],germinateindifferentconcentrationsofsaltstresswereNaClsingleseedgerminationexperiment(concentrationgradientfor0,0.2%,0.4%0.6percent,andsevengradient,1.0%,1.2%),thedeterminationofgerminationrate,germinationindex,potentialandvigorindex,index.The
4、resultsshowedthatthesingleundersaltstressconcentrationofstress,alongwiththeincreaseofseedgermination,rape,germinationindex,germinationandvigorindexhaddifferentdegrees,relativerateofsalt,seedstogerminatetimedelayandgerminationprocess,Thestressonrapeseed
5、NaClonseedgermination,includingtheinhibitionofthestressconcentrationofsoybeanNaCl0.4%ratereachedtheinhibitionof50%respectively,concentration,88%88forNaClof2.0%underthestressoftwoselectedmaterialsforthegerminationrate,Saltstressonseedgermination,butlowc
6、oncentrationofsaltstressonseedgermination.ShengHongrapeseedthanitsrapeseedsaltresistance.Keywords:sodiumchloridestress,Rape,Seedgermination目录摘要...................................................................1Abstract.................................
7、..............................21.前言.................................................................21.1油菜概述............................................................21.2研究的目的和意义......................................................31.3国外研究的现状.......
8、...............................................32.材料与方法...........................................................42.1试验材料............................................................42.2试验药品..............................................