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时间:2020-05-20
《阿司匹林对宫颈癌裸鼠移植瘤生长的影响及Bax与VEGF的表达变化-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、第34卷第6期西安交通大学学报(医学版)Vo1.34No.62013年11月JournalofXi’anJiaotongUniversity(MedicalSciences)Nov.2O13阿司匹林对宫颈癌裸鼠移植瘤生长的影响及Bax与VEGF的表达变化李龙,尚卫华。,赵娟,王月玲(1.西安交通大学医学院第一附属医院妇产科,陕西西安710061;2.西安医学院附属医院急诊外科,陕西西安710077)摘要:目的探讨阿司匹林对宫颈癌裸鼠移植瘤生长的抑制作用及可能机制。方法选取裸鼠4O只,随机分为空白对照组、阿司匹林组、顺铂(DDP)组及阿司匹林联合DDP组。各组裸鼠均
2、在其背部皮下注射HeIa细胞,18d后处死,期间观察各组出瘤时间,分别检测移植瘤质量及体积并计算抑瘤率,免疫组化法检测移植瘤组织中Bax及血管内皮生长因子(VEGF)的表达。结果阿司匹林组与对照组比较,裸鼠出瘤时间延长,肿瘤体积及质量均减少(P<0.05);阿司匹林联合DDP组裸鼠肿瘤生长最慢,经两因素析因分析,P<0.05,两药联合应用具有交互效应;阿司匹林组Bax蛋白表达上调,与对照组相比差异具有统计学意义(P<0.05),阿司匹林联合DDP组对Bax上调的作用最强,与单用DDP组相比较,差异具有统计学意义(P3、照组相比差异具有统计学意义(P4、thenudemouseandtheexpressionsofBaxandVEGFLILong,SHANGWei—hua。,ZHAOJuan,WANGYue—ling(1.DepartmentofGynecology8LObstetrics,theFirstAffiliatedHospital。MedicalSchoolofXi’anJiaotongUniversity,Xi’an710061;2.DepartmentofEmergencySurgery,theAffiliatedHospital,Xi’anMedicalCollege,Xi’an710077,C5、hina)ABSTRACT:ObjectiveToinvestigatetheinhibitoryeffectofaspirinonthegrowthofcervicaltransplantedtumorinthenudemouseanditspossiblemechanism.MethodsFortynudemicewereselectedandrandomlydividedintofourgroups:conventionalsalinetreatment,aspirintreatment,dichlorodiammineplatinum(DDP)treatm6、ent,andaspirin+DDPtreatment.MiceofallgroupswerethensubjectedtoasubcutaneousinjectionofHeLacellsinthebackandkilled18daysafterinjection.Timeoftumorgrowthandtumorweightweredetected;theinhibitionrateoftumorwascalculated;Baxandvascularendothelialgrowthfactor(VEGF)expressionswereexaminedbyi7、mmunohistochemistry.ResultsThetimeoftumorgrowth,tumorvolumeandweightwerereducedinaspiringroupcomparedwiththecontrolgroup(P<0.05).Tumorgrowthwastheslowestinaspirin+DDPgroup,andthetwo—factorfactorialanalysisshowedthatthetwodrugsincombinationhadaninteractiveeffect(P<0·05)-TheBaxproteinwa8、sup—r
3、照组相比差异具有统计学意义(P4、thenudemouseandtheexpressionsofBaxandVEGFLILong,SHANGWei—hua。,ZHAOJuan,WANGYue—ling(1.DepartmentofGynecology8LObstetrics,theFirstAffiliatedHospital。MedicalSchoolofXi’anJiaotongUniversity,Xi’an710061;2.DepartmentofEmergencySurgery,theAffiliatedHospital,Xi’anMedicalCollege,Xi’an710077,C5、hina)ABSTRACT:ObjectiveToinvestigatetheinhibitoryeffectofaspirinonthegrowthofcervicaltransplantedtumorinthenudemouseanditspossiblemechanism.MethodsFortynudemicewereselectedandrandomlydividedintofourgroups:conventionalsalinetreatment,aspirintreatment,dichlorodiammineplatinum(DDP)treatm6、ent,andaspirin+DDPtreatment.MiceofallgroupswerethensubjectedtoasubcutaneousinjectionofHeLacellsinthebackandkilled18daysafterinjection.Timeoftumorgrowthandtumorweightweredetected;theinhibitionrateoftumorwascalculated;Baxandvascularendothelialgrowthfactor(VEGF)expressionswereexaminedbyi7、mmunohistochemistry.ResultsThetimeoftumorgrowth,tumorvolumeandweightwerereducedinaspiringroupcomparedwiththecontrolgroup(P<0.05).Tumorgrowthwastheslowestinaspirin+DDPgroup,andthetwo—factorfactorialanalysisshowedthatthetwodrugsincombinationhadaninteractiveeffect(P<0·05)-TheBaxproteinwa8、sup—r
4、thenudemouseandtheexpressionsofBaxandVEGFLILong,SHANGWei—hua。,ZHAOJuan,WANGYue—ling(1.DepartmentofGynecology8LObstetrics,theFirstAffiliatedHospital。MedicalSchoolofXi’anJiaotongUniversity,Xi’an710061;2.DepartmentofEmergencySurgery,theAffiliatedHospital,Xi’anMedicalCollege,Xi’an710077,C
5、hina)ABSTRACT:ObjectiveToinvestigatetheinhibitoryeffectofaspirinonthegrowthofcervicaltransplantedtumorinthenudemouseanditspossiblemechanism.MethodsFortynudemicewereselectedandrandomlydividedintofourgroups:conventionalsalinetreatment,aspirintreatment,dichlorodiammineplatinum(DDP)treatm
6、ent,andaspirin+DDPtreatment.MiceofallgroupswerethensubjectedtoasubcutaneousinjectionofHeLacellsinthebackandkilled18daysafterinjection.Timeoftumorgrowthandtumorweightweredetected;theinhibitionrateoftumorwascalculated;Baxandvascularendothelialgrowthfactor(VEGF)expressionswereexaminedbyi
7、mmunohistochemistry.ResultsThetimeoftumorgrowth,tumorvolumeandweightwerereducedinaspiringroupcomparedwiththecontrolgroup(P<0.05).Tumorgrowthwastheslowestinaspirin+DDPgroup,andthetwo—factorfactorialanalysisshowedthatthetwodrugsincombinationhadaninteractiveeffect(P<0·05)-TheBaxproteinwa
8、sup—r
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