双氢青蒿素对前列腺癌裸鼠种植瘤生长及vegf表达的影响

《双氢青蒿素对前列腺癌裸鼠种植瘤生长及vegf表达的影响》由会员上传分享，免费在线阅读，更多相关内容在工程资料-天天文库。

1、双氢青蒿素对前列腺癌裸鼠种植瘤生长及VEGF表达的影响李庆春高小玲石之虎邹聪罗子国（重庆医科大学电镜室，重庆400016）摘要目的研究双氢青蒿素（DHA）对前列腺癌PC-3细胞裸鼠种植瘤的抑制作用及对VEGF表达的影响，并探讨其作用机制。方法采用人前列腺癌PC-3细胞建立裸鼠种植瘤模型，20只模型鼠随机分为对照组、溶剂组、DHA大剂量组及DHA小剂量组，经13d干预后，计算抑瘤率；光镜及电镜观察肿瘤组织形态学表现，免疫组织化学法检测VEGF的表达水平，体视学分析肿瘤微血管密度（MVD）。结果DHA大小剂量组抑瘤率分别为69.221%和63.186%，肿瘤组织内凋亡细

2、胞明显增多，可见典型的凋亡小体；免疫组化结果显示，DHA大小剂量组VEGF表达及MVD均低于对照组（P＜0.05）。结论DHA具有较强的抗肿瘤作用，其机制与下调凋亡相关基因VEGF有关。关键词:双氢青蒿素；前列腺癌；凋亡；VEGF；微血管密度中图法分类号：R284.1;R979.1文献标识码：ATheeffectsofdihydroartemisininontransplantedprostatecancerinnudemiceandexpressionofVEGFLIQing-chun,GAOXiao-lin,SHIZhi-hu，ZOUCong,LUOZi-guo

3、*(LaboratoryofElectronMicroscope,ChongqingMedicalUniversity,Chongqing400016,China)[Abstract]Objective:Tostudytheinhibitoryeffectsofdihydroartemisinin(DHA)onthegrowthoftransplantationhumanprostatetumorPC-3cellsinnudemiceandtheexpressionlevelofVEGFandexploretheunderlyingactionmechanismof

4、DHA.Methods:ProstatecancerPC-3cellsweretransplantedinto20nudemicetoestablishthesolidtumormode.Thesenudemicewererandomlydividedinto4groupswith5miceineachgroup:controlgroup,solventgroup,largedoseDHAgroupandlowdoseDHAgroup.Thetumorgrowthinhibitionratewascalculatedonday13afterdrugadministr

5、ation;PathomorphismchangesofPC-3cellswereobservedbylightmicroscope(LM)andtransmissionelectronmicroscope(TEM)afteradministrationofDHA.TheproteinexpressionofVEGFwastestedbyimmunohistochemicalmethod;作者简介：李庆春，河北省张家口市人，男，医师，主要从事前列腺癌方面的研究。电话:13658380331，E-mail:695786349@qq.com通信作者：罗子国，电话：（02

6、3）68485838，E-mail:luoziguo@cqmu.edu.cn收稿日期：08-07-11修回日期：08-08-31Micro-vasvulordensity(MVD)wasanalysedbystereologicalmethod.Results:ThetumorgrowthinhibitionratesoflargedoseDHAgroupandlowdoseDHAgroupwere69.221%and63.186%;ApoptoticcellsweresignificantlyincreasedandTEMexaminationrevealedth

7、atthescatteredapoptoticbodieswereobservedintumortissuesofDHAgroups;ImmunohistochemicalexaminationrevealedthattheproteinexpressionofVEGFwasdecreasedinDHAgroups（p＜0.05）;MVDwassignificantlydecreasedinDHAgroups（p＜0.05）.Conclusion:TheseresultsdemonstratedthatDHAhasstrongerinhibitoryeffect