猕猴桃液体授粉花粉液制备技术研究.pdf

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1、中圊患亏c亟搪2015,31(7)：86-90ChineseAgriculturalScienceBulletin猕猴桃液体授粉花粉液制备技术研究陈建业，李占红，宁玉霞(许昌职业技术学院园林园艺系，河南许昌461000；许昌林科所，河南许昌461000)摘要：为建立猕猴桃液体授粉花粉悬浊液的高效制备方法，以猕猴桃混合雄株花粉和‘豫猕猴桃2号’为试材，进行了利用粗制花粉配制花粉悬浊液及液体授粉试验。结果表明，悬浊液的花粉浓度随粗制花粉添加量的增大而增加；ka制花粉添加量12％-16％(w／v)时，悬浊液的花

2、粉浓度可达125万~MmL-175万粒／mL，花粉发芽率85％以上，与对照(添加5％精制花粉)无显著性差异；各处理坐果率几乎均为100％；12％以上处理单果重100g左右，种子数l100～1220粒，与对照无显著性差异；4％处理单果重、种子数则显著低于以上处理；不同处理间可溶性固形物含量和酸含量无显著性差异。吸光指数与花粉浓度具有密切线性相关关系r=0．98)，吸光指数2．5可作为判断花粉浓度是否适宜的指标。该法省去了花粉精制程序，是一种高效省力的猕猴桃人工授粉技术。关键词：猕猴桃；‘豫猕猴桃2号’；喷雾

3、授粉；花粉悬浊液；制备方法中图分类号：$663．4文献标志码：A论文编号：2014．2112StudyonthePreparationTechnologyofPollenSuspensionLiquidforSprayPollinationofActinidiachinensisChenJianye，LiZhanhong，NingYuxia(DepartmentofLandscape&Horticulture，XuchangVocationalTechnologyCollege，XuchangHenan4

4、61000；2XuchangForestryInstitute，XuchangHenan461000)Abstract：Todeveloptheeffectivepreparationmethodsofpollensuspensionforkiwispraypollination，theauthortookthemixedpollenand‘Yumihoutao2’asmaterials，andcarriedoutthestudybyusingcrudepollenforpreparationofpoll

5、ensuspensionandthetestofliquidpollination．Thetestresultsshowedthatthepollenconcentrationofthesuspensionincreasedalongwiththeincreaseofcrudepollenaddedamount．Whencrudepollenaddingamountwas12％一16％(w／v)，thepollenconcentrationsofthesuspensionwasupto1．25—1．75m

6、illion／mL，andthepollengerminationratewasmorethan85％，andtherewasnosignificantdifferencewithcontrolledtrials(add5％ofrefinedpollen)，andthefruitsettingratewasalmost100％undereachtreatment．Withcrudepollenaddingamountofmorethan12％，thetreatedfruitweighedabout100g

7、，theseednumberwas1100-1220，andtherewasnosignificantdifierenceincomparingwiththecontrolledtrials．Fruitweightandseednumberoftreatmentwith4％crudepollenaddingamountweresignificantlylowerthanthatoftheabovetreatments．Forsolublesolidscontentandacidcontent，therew

8、asnosignificantdifferencebetweendifferenttesttreatments．Lightabsorptionindexofsuspensionhadaclosecorrelationrelationshipwithpollenconcentration(r=0．98)，andabsorptionindexof2．5couldbeusedasanindexforjudgingpollenconc