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《山药多糖对PRRSV灭活苗免疫猪抗体和T细胞亚群的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、-BT●8勰骺华北农学报·2010,25(2):236-238山药多糖对PRRSV灭活苗免疫猪抗体和T细胞亚群的影响张红英,王学兵,崔保安,赵现敏,陈红英,金钺(1.河南农业大学牧医工程学院,河南郑州450002;2.河南省动物性食品安全重点实验室,河南郑州450002)摘要:研究山药多糖对PRRSV灭活苗免疫抗体和猪外周血T细胞亚群的影响。选择35日龄断奶仔猪l6只,随机分为4组,将山药多糖分别以17.1mr,/kg和8.55ms/kg两个剂量和猪PRRSV灭活苗同时注射,于免疫后7,14,24,34,44,54,69,79d采血,ELI
2、SA方法检测猪PRRSV抗体水平,流式细胞术检测猪外周血中T淋巴细胞亚群的变化。结果表明,山药多糖可显著提高PRRSV灭活苗免疫猪外周血CD3和CD8细胞数量,在免疫34d后,两个剂量的山药多糖均可显著提高PRRSV灭活苗免疫抗体水平。山药多糖可以作为免疫增强剂与PRRSV灭活苗联合使用。关键词:山药多糖;PRRSV灭活苗;抗体;T淋巴细胞亚群中图分类号:$852.65文献标识码:A文章编号:1000—7091(2010)02—0236—03EffectsofChineseYamP0lvsaccharideonAntibodyandTLym
3、phocytesSubpOpulatiOnsintheImmuneResponsetoPRRSVKilledVaccineZHANGHong—ying’,WANGXue—bing,,CUIBao—an,,ZHAOXian.min,CHENHong—ying‘,JINYue(1.CollegeofAnimalHusbandryandVeterinaryMedicine,HenanAgriculturalUniversity,Zhengzhou450002,China;2.HenanKeyLaboratoryforAnima1.derivedF
4、oodSafety,Zhengzhou450002。China)Abstract:TheresearchistostudytheeffectsofChineseyampolysaccharide(CYPS)onantibodyandTsubpopu-lationsinpigletspheripheralbloodintheimmuneresponsetoPRRSVkilledvirusvaccine.1635·day-oldpigletswererandomlyassignedintoequalfourgroupsandthreegroup
5、swerevaccinatedwithPRRSVkilledvirusvaccine.Atthesametime,thepigletsingroupsCYPSHandCYPSLwereintramuscularlyinjectedwithCYPSat17.1or8.55mg/kgdosages,groupC(controlgroup),withsterilesaline,onceadayforthreesuccessivedays,groupF(fakegroup)onlyinjectedwithsaline.At7,14,24,34,44
6、,54,69,79dayaftervaccination,pheripheralbloodweresampledfordetectionofserumantibodytitertoPRRSVbyELISAandTlymphocytessubpopulationsbycytofluorometry.ResultsshowedthatCYPScanboosttheproportionofCD3andCD8lymphocytesinpigletspheripheralbloodafterimmunizationwithPRRSVkilledvir
7、usvaccine,andenhancedantibodytitertoPRRSVafter34days.CYPSisapotentialimmunostimula-toryforPRRSVkilledvirusvaccine.Keywords:CYPS;PRRSVkilledvirusvaccine;Antibody;Tlymphocytesubpopulations山药为薯蓣科多年生宿根蔓草植物山药(D—免疫力¨’,但山药多糖对猪是否同样具有免疫促oscoresoppositaThunb)的块根,又名白苕、土薯、大进作用尚未见报道。薯、
8、薯药,具有补脾、益肺、固肾、益精的作用,原名薯猪繁殖与呼吸综合征(Porcinereproductiveand蓣,首见于《神农本草经》。据《本草纲目》记载,山respirator
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