黄参多糖提取及其对雏鸡外周血T淋巴细胞增殖的影响.pdf

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1、J．SHANXIAGRIC．UNIV．(NaturalScienceEdition)雹—圜唧l学榴r自然科学版)2o15．35(2)：133003230黄参多糖提取及其对雏鸡外周血T淋巴细胞增殖的影响赵有淑。，孙宝，方梅，贾宁¨(1．甘肃农业大学动物医学院，甘肃兰州730070；2．上海海利生物技术股份有限公司，上海200000)摘要：为探讨黄参多糖(sGP)的提取纯化和免疫活性作用，采用微波处理法从黄参中提取制备SGP090％微波处理9Omin，过滤，离心，浓缩，用酒精沉淀出粗多糖，冷冻干燥机中冻干，多糖精制，理化性质

2、鉴定。采用MTT法比较不同作用浓度的SGP对鸡外周血T淋巴细胞体外增殖作用的影响。结果表明，SGP得率在微波处理90min时，黄参多糖得率高达5．85。理化性质鉴定和红外光谱分析，证明提取的SGP纯度较好，不舍淀粉、氨基酸或蛋白质。外周血T淋巴细胞增殖试验表明，SGP能够刺激和促进雏鸡淋巴细胞增殖，并且能协同ConA(刀豆素A)刺激雏鸡外周血T淋巴细胞增殖。并且，上述2种刺激作用都具有一定的剂量效应关系，均以SGP终作用浓度在250mg·I时效果最佳。SGP具有免疫增强作用。关键词：黄参多糖；提取；雏鸡；T淋巴细胞；增殖

3、中图分类号：Q55；R739．8文献标识码：A文章编号：1671—8151(2015)02—0133—04StudyonExtractionofSphailerocarpusGracilisP0lysaccharides(SGP)andtheEffectofSGPonPe—ripheralTLymphocyteProliferationinChicksZhaoYoushu，SunBao’，FangMei’，JiaNing(1．CollegeofVeterinaryMedicine，GansuAgriculturalUni

4、versity，LanzhouGansu730070，China；2．ShanghaiHai—liBiologicalTechnologyIncorporatedCompany，Shanghai200000，China)Abstract：Tostudytheextraction，purificationandimmunocompetenceofSphallerocarpusGracilispo1ysaccharides(SGP)，theSGPwasextractedwithmicrowaveprocessingfor90

5、minutesat90％，filtrated，centrifuged，condensed，anddepositingthecrudepolysaccharideswithalcohol，dried，refinedandidentification．TheeffectsofSGPonperiph—eralTlymphocyteproliferationinchickswasdeterminedbyMTTmethod．Theresultsshowedthatwhentheextrac—tionconditionofmicro

6、wavewas90min，theyieldofSGPwas5．85A0．TheSGPyieldwassignificantlyhigherwithtreatedbymicrowaveprocessingandfreezedehydrationtoobtainthepolysaccharide．TheresultsofidentificationofphysiochemicalpropertyandinfraredspectroscopyprovedthatthepurityofSGPwasgood，nostarch，am

7、inoacidsorproteins．ThedatashowedthatSGPcouldpromotelymphocyteproliferationinchicks．AnditalsocouldcooperatewithConAtOpromoteperipheralbloodlymphocyteproliferationinchicks．Furhteranalysisshowedthat，botheffectsrelatedtOitsdosage。themosteffectivedoseoftheSGPconcentra

8、tionis250mg·L一．TheSGPcanimprovetheimmunocom—petence．Keywords：SphaIlerocarpusGracilispolysaccharides(SGP)；Extraction；Chicks；Tlymphocyte；Proliferation黄参是伞形科(Umbe