胶孢炭疽菌侵染柱花草叶片的显微观察.pdf

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1、第24卷第5期草业学报2015年5月Vo1．24，No．5ACTAPRATACULTURAESINICAMay，2015DOI：10．1l686／cyxb20150521http：／／eyxb．Izu．edu．cn杨茂霞，林国彪，陈彩虹，王荟，罗丽娟．胶孢炭疽菌侵染柱花草叶片的显微观察．草业学报，2015，24(5)：175—181．YangMX，LinGB，ChenCHWangH，I110LJ．MicroscopicobservationofStyloinfectedbyColletotrichumgZo0sporodPs．ActaPra

2、taculturaeSinica，2015，24(5)：175-181．胶孢炭疽茵侵染柱花草叶片的显微观察杨茂霞，林国彪，陈彩虹，王荟，罗丽娟(海南大学农学院，海南省热带生物资源可持续利用重点实验室，海南海口570228)摘要：为了研究胶孢炭疽菌在柱花草叶片上的侵染进程和植株接种病原菌后的反应，用胶孢炭疽菌孢子悬浮液接种生长6周的热研2号柱花草幼苗，显微观察接种后炭疽菌在柱花草叶片上的侵染过程，统计植株病情指数。结果表明，接种4h时孢子开始萌发、少量形成附着胞；6h孢子大量萌发，12h时大量附着胞形成并形成侵染钉；36h时开始产生菌丝；48

3、h时菌丝大量产生纠结成网状；60h时开始形成分生孢子梗；72h时产生新的分生孢子。接种72h部分病原菌完成了一个侵染循环。同时柱花草发病症状为：叶片24h黄化，有侵染点细胞膜破裂，48h出现褐色病斑，72h病斑出现黑褐色坏死线、点状菌核、绒毛状菌丝，96h细胞崩解死亡，组织液外流。8d病情指数为54．52，11d有55的接种植株死亡。关键词：柱花草；柱花草炭疽菌；侵染；显微结构；病害症状MicroscopicobservationofstlosnntheseinfectedbyColletotrichumgloeosporioidesYAN

4、GMao—Xia，LINGuo—Biao，CHENCai—Hong，WANGHui，LUOLi—JuanAgronomyofHainanUniversity，HainanProvincialKeyLaboratoryofTropicalBiologicalResourcesSustainableUtilization，Haikou570228，ChinaAbstract：InordertostudythediseaseprogressofStyloanthrocnoseandtheinoculatedplantsreaction，the6w

5、eeksoldofStylosantheseguianensisCV．Reyan2inoculatedbythesuspensionliquidofColletotrichumgloeos—porioidesspores．Microscopicobservationsoftheleavesrevealedtheinfectingprocessandcountingthediseaseindexthroughplantsymptoms．Sporesgerminatedandafewsporesformedappressoriumat4h．Mo

6、stofsporesgerminatedat6h，andalargequantityofgeminatingsporesformedappressoriumat12handbegantoformpenetrationpegs．Theinoculatedsporesbegantoproducehyphaeat36h，alotofhyphaeformedandstaggeredintoameshonthesurfaceoftheleaveat48h．Thegeminatingsporesbegantoformconidiophoresat60h

7、．Numerousofnewsporesbegantoproduceat72h．Partsofinoculatedsporeshavefinisheddiseasecyclein72h．Inthesametime，plantsappeareddiseasesymptomgradually：leavescolorbecameyellow，thehostcellmembraneruptureat24h，brownnecrosisappearedat48h，thendeadline，sclerotinite，myceliumappearedont

8、heleavessurfaceat72h．Thehostcellbegantocollapseat96h．Diseaseindexis54．52at8days，55ofinocu