分子标记技术在咖啡育种上的应用(英文).pdf

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1、nCT^nBRICULTURnE华北农学报·2008，23(增刊)：i29．132B0REnLl—SIHICn分子标记技术在咖啡育种上的应用李国鹏，何红艳，黄家雄，罗心平，李贵平，杨仕贵，李树维，黄健，郭容琦(云南省农科院热带亚热带经济作物研究所，云南保山678025)摘要：主要介绍了AFLP和RAPD2种分子标记技术以及2种分子标记的优缺点。同时，着重介绍了2种分子标记在咖啡育种上的应用成果。在最后还列举了2个其他的分子标记在咖啡育种上的应用。关键词：咖啡；分子标记技术；AFLP；RAPD；应用中图分类号：Q75文献标识码：A文章编号：1000—7091(2008)~刊一0129—

2、04MoleculeMarkerTechnologyinCoffeeBreedingApplicationLIGuo—peng，HEHong—yan，HUANGJia—xiong，LUOXin—ping，LIGui—ping，YANGShi—gui，LIShu—wei，HUANGJian，GUORong—qi(TropicalandSubtropicalCashCropsInstituteofYunnanAgricultureScienceResearchInstitute，Baoshan678025，China)Abstract：ThisarticleintroducedAFLPa

3、ndRAPDtechnologies，anditsapplicationincofeebreedingprogram．ThisarticleintroducedthemeritanddisadvantagesoftheAFLPandRAPD．Attheend，gave2samplesofothermoleculemarkertechnologyincoffeebreeding．Keywords：Coffee：Moleculemarkertechnology；AFLP；RAPD；ApplicationCofleeandteaarethetwomostimportantbeverages

4、Inrecentyears，DNAmolecularmarkerssuchasoftheworld．AlthoughthegenusCoffeecontainsabout80AFLP，RAPDandISSR，whichareindependentofenvi—taxa，commercialcofeeproductionisbasedontwospeciesronmentalinfluences，havebeenusedinthecoffeebreed．i．e．C．arabica(2n=4x=44)andC．canephora(2n：ingresearch．Inthisarticle，

5、wejustintroducedtheprinci—2x=22)．Theyaccountingrespectivelyforabout70％andplesofsomemodernbio—technologiesanditsapplication30％oftheworldproduction．TheArabicacofeecultiva—onthecoffeebreedingresearch．tionstartedinArabia，specificallyinYemen，fivecenturies1Thebriefintroductionandutilizationofago．Inth

6、eearly18thcentury，progeniesfromasingleIn—AFLPincoffeebreedingdonesiaplantcultivatedinEuropewerespreadouttoAmplifiedFragmentLengthPolymorphisms(AFLP)SouthAmericaandturnedouttobethegeneticbasisofisbasedontheselectivePCRamplificationofrestrictionmaincuhivamofBrazilandothercountries．Severalcen·frag

7、mentsfromatotaldigestofgenomicDNA．AndAFLPturiesago，theaimofcropbreedingjustemphasisonhighisaDNAfingerprintanalysismethodwithhighdiscrimi—yield．Nowadays，thebreedingprogramarecenteringonnatorycapacity．Comparedtoothermarkertechniques