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时间:2020-05-09
《雌激素缺乏对大鼠核因子C(Nfic)与成骨相关基因表达的影响.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、口腔颌面修复学杂志2015年5月第16卷第3期·论著·雌激素缺乏对大鼠核因子C(Nfic)与成骨相关基因表达的影响术李传洁刘娜王俊成鄂玲玲魏泽宁李亚男【摘要】目的:通过检测分析骨质疏松大鼠股骨组织核因子C(NuclearFactorI-c,Nfic)与成骨相关基因表达情况,探讨雌激素缺乏状况下两类基因表达的相关性,探寻雌激素相关的骨质疏松发生原理。方法:3月龄未交配雌性SD大鼠2O只,随机分为两组:A、假去势手术组(SHAM);B、去势手术组(ovx)。对A组大鼠行假去势手术,B组大鼠行去势手术。去势手术前、去势手术后1、2、3、4、5、
2、6周及处死前称重,观察大鼠体重变化。卵巢切除术后1.5个月,采用双能骨密度测量仪测量大鼠腰椎及股骨远中骨密度,处死后,取双侧后肢股骨进行Q-PCR,检测核因子C(Nfic)、核心结合因子2(Runx2)、碱性磷酸酶(Alp)的mRNA表达情况。结果:去势手术前,两组大鼠体重差异无统计学意义(尸>0.05),术后2、4、6周,OVX组大鼠体重较SHAM组显著增加(P<0.05,PO.05);术后1.5个月,0VX组大鼠腰椎及左股骨骨密度较SHAM组显著降低(P<
3、0.01,P<0.05)。去势组大鼠的Nfic、Runx2、Alp基因表达量明显低于假去势手术组(尸<0.05)。结论:雌激素缺乏大鼠较正常组肥胖,骨密度减低,骨质改变,成骨能力减弱,成脂功能提高;Nfic基因与Runx2、Alp成骨相关基因表达量呈正相关。关键词:绝经后;骨质疏松;雌激素;核因子C;核心结合因子2;碱性磷酸酶;mRNA[中国图书分类号】R78.2【文献标识码]A[文章编号]1009—3761(2015)03—0l29一O5EfectsofestrogendeficiencyontheexpressionofNficand
4、osteogenicgeneLlChuan一uUNa,wANGJun—cheng.ELing—lingWEIZe-ning。LIYa-nan.(MedicalResearchCenter,ChinesePLAGeneralHospital,Beijing100853.China)[Abstract]Objective:ToinvestigatetheexpressionofNficandosteogenicgeneofthefemurinosteoporosisratsinor-dertoexplorethecorrelationbetw
5、eenthesegeneexpressioninlackofes~ogenandtheprincipleofes~ogenrelatedosteo-porosis.Methods:3-montholdunmatedfemaleSDratswererandomlydividedintotwogroups:GroupA(SHAMgroup,10rats)andGroupB(ovariectomizedgroup,ovx)(10rats).Bodyweightwasrecordedbeforeoperation,1,2,3,4,5,6weeks
6、afteroperationandbeforesacrifice.Thebonemineraldensityofthelumbarspineandthedistalfemurwithdualen-ergyabsorptiometryweremeasured1.5monthsafteroperation.ThemRNAexpressionofNuclearFactorI-c(Nfie),runt-relatedtranscriptionfactor2(Runx2)andalkalinephosphatase(ALP)byQ—PCRafter
7、sacrificedwerealsorecorded.Results:Thereisnosignificantdiferenceinweightbetweenthethreegroupsofra~(P>0.05)beforeoperation;theweightinOVXgroupincreasedsignificantlycomparedtoSHAMgroup(P<0.05,P8、isticaldiferencebetweenthetwogroupsbeforeoperation(尸>0.O5);1.5monthsafteroperation,theboneminera
8、isticaldiferencebetweenthetwogroupsbeforeoperation(尸>0.O5);1.5monthsafteroperation,theboneminera
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