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时间:2020-05-07
《PS1dE9双转基因阿尔茨海默病模型小鼠肝叶部分切除术后认知功能及海马内Cdk5、p25和p35表达的影响-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、临床麻醉学杂志2014年7月第30卷第7期JClinAnesthesiol,July2014,Vo1.30,No.7·693·.实验研究.雷公藤红素对APPswe/PS1dE9双转基因阿尔茨海默病模型小鼠肝叶部分切除术后认知功能及海马内Cdk5、p25和p35表达的影响许方方万燕杰徐静【摘要】目的动态观察雷公藤红素对APPswe/PS1dE9双转基因阿尔茨海默病(alzheimersdisease,AD)模型小鼠肝叶部分切除术后认知功能及海马内周期蛋白依赖性蛋白激酶5(cyclin-de—pendentkinase5,Cdk5)和激动因子p25
2、和p35表达的影响。方法3月龄AD模型小鼠96只,随机均分为三组:手术组(S组),麻醉后行肝叶部分切除术;雷公藤红素组(C组),手术前3d上午腹腔注射雷公藤红素60>g/kg;二甲亚枫(DMSO)组(0组),手术前3d上午腹腔注射同体积的0.04DMSO。三组随机抽取8只小鼠采用水迷宫连续训练5d并检测术后1、3、7、14d的学习记忆能力,余下小鼠分别于术后1、3、7、14d取标本,每次6只,检测海马内细胞Cdk5及激动因子p25、p35的表达。结果与C组比较,术后3、7和14dS和。组小鼠逃避潜伏期明显延长(P3、明显降低(P4、e/PSldE9doubletransgenicmorseafterpartialhepatectomyXUFang-fang。14Yan-jie。XUJing.eDepartmentofPain,GonglHospital,Pudong,Shanghai200135,ChinaCorrespondingauthor:4ⅣYan—jie,Email:yaie—wan@sina.corn[Abstract]ObjectiveToinvestigatetheimpactofcelastroloncognitivefunctionandtheex—p5、ressionofCdkS/p25inhippocampusinAPPswe/PSldE9doubletransgenicmouseafterpartialhepa—tectomy.MethodsThree-month—oldAPPswe/PSldE9doubletransgenicmice(,z一96)wererandomlydividedintosurgerygroup(partialhepatectomy,groupS),celastrolgroup(celastrolandpartialhepa—tectomy,groupC)andDM6、SOgroup(DMS0andpartialhepatectomy,group0).Eightmicewerese一1ectedrandomlyineachgroupandwereMorris—watermazetrainedfor5continuousdays.Theirlearn—ingandmemoryabilitieswereevaluatedat1,3,7and14dafterthesurgery,respectively.Fortheremainingmiceineachgroup,thehippocampuswerecollect7、edandthechangesofCdk5,p35andp25inhippocampusweremeasuredbywesternblotatthetime1,3,7,14dafterpartialhepatectomy.Sixmicewerekilledateachtimefordatacollection.ResultsTheaverageescapelatencyofgroupCwassignificantlyshorterthanthoseofgroupsSandOat3,7and14dafterpartialhepatectomy(P8、<0.05).ThepercentagesoftimeintargetquadrantofgroupsSandOdecreasedsignifican
3、明显降低(P4、e/PSldE9doubletransgenicmorseafterpartialhepatectomyXUFang-fang。14Yan-jie。XUJing.eDepartmentofPain,GonglHospital,Pudong,Shanghai200135,ChinaCorrespondingauthor:4ⅣYan—jie,Email:yaie—wan@sina.corn[Abstract]ObjectiveToinvestigatetheimpactofcelastroloncognitivefunctionandtheex—p5、ressionofCdkS/p25inhippocampusinAPPswe/PSldE9doubletransgenicmouseafterpartialhepa—tectomy.MethodsThree-month—oldAPPswe/PSldE9doubletransgenicmice(,z一96)wererandomlydividedintosurgerygroup(partialhepatectomy,groupS),celastrolgroup(celastrolandpartialhepa—tectomy,groupC)andDM6、SOgroup(DMS0andpartialhepatectomy,group0).Eightmicewerese一1ectedrandomlyineachgroupandwereMorris—watermazetrainedfor5continuousdays.Theirlearn—ingandmemoryabilitieswereevaluatedat1,3,7and14dafterthesurgery,respectively.Fortheremainingmiceineachgroup,thehippocampuswerecollect7、edandthechangesofCdk5,p35andp25inhippocampusweremeasuredbywesternblotatthetime1,3,7,14dafterpartialhepatectomy.Sixmicewerekilledateachtimefordatacollection.ResultsTheaverageescapelatencyofgroupCwassignificantlyshorterthanthoseofgroupsSandOat3,7and14dafterpartialhepatectomy(P8、<0.05).ThepercentagesoftimeintargetquadrantofgroupsSandOdecreasedsignifican
4、e/PSldE9doubletransgenicmorseafterpartialhepatectomyXUFang-fang。14Yan-jie。XUJing.eDepartmentofPain,GonglHospital,Pudong,Shanghai200135,ChinaCorrespondingauthor:4ⅣYan—jie,Email:yaie—wan@sina.corn[Abstract]ObjectiveToinvestigatetheimpactofcelastroloncognitivefunctionandtheex—p
5、ressionofCdkS/p25inhippocampusinAPPswe/PSldE9doubletransgenicmouseafterpartialhepa—tectomy.MethodsThree-month—oldAPPswe/PSldE9doubletransgenicmice(,z一96)wererandomlydividedintosurgerygroup(partialhepatectomy,groupS),celastrolgroup(celastrolandpartialhepa—tectomy,groupC)andDM
6、SOgroup(DMS0andpartialhepatectomy,group0).Eightmicewerese一1ectedrandomlyineachgroupandwereMorris—watermazetrainedfor5continuousdays.Theirlearn—ingandmemoryabilitieswereevaluatedat1,3,7and14dafterthesurgery,respectively.Fortheremainingmiceineachgroup,thehippocampuswerecollect
7、edandthechangesofCdk5,p35andp25inhippocampusweremeasuredbywesternblotatthetime1,3,7,14dafterpartialhepatectomy.Sixmicewerekilledateachtimefordatacollection.ResultsTheaverageescapelatencyofgroupCwassignificantlyshorterthanthoseofgroupsSandOat3,7and14dafterpartialhepatectomy(P
8、<0.05).ThepercentagesoftimeintargetquadrantofgroupsSandOdecreasedsignifican
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