慢性HBV感染者外周血单个核细胞分泌干扰素α功能初探-论文.pdf

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1、l7·临床论著·慢性HBV感染者外周血单个核细胞分泌干扰素功能初探程兆晶周宝桐张丽帆侍效春韩扬张月秋徐虹刘晓清【摘要】目的旨在检测慢性HBV感染者外周血单个核细胞(PBMC)分泌干扰素Ⅱ(IFN-a)的情况，以探究其外周血浆细胞样树突状细胞(pDC)和单核细胞功能是否存在缺陷。方法前瞻性纳入2012年7月至2013年3月北京协和医院肝炎门诊就诊的慢性乙型肝炎(CHB)患者I6例，同期选取与之年龄相匹配的HBV携带者16例和健康人18例。采集血样，分离PBMC，分别与含未甲基化的胞嘧啶鸟嘌呤二核苷酸序列的寡脱氧核苷酸2216(CPGODN2216)、聚肌苷酸胞苷酸(

2、polyI：C)刺激共培养，并用酶联免疫吸附试验(ELISA)测量其分泌IFN-q量。结果经ODN2216刺激后，CHB组患者PBMC分泌IFN．a量平均为31．2O(7．33~44．04)pg／ml，HBV携带者组PBMC分泌IFN一0【量平均为109．91(I3．74~240．27)pg／ml，健康对照组PBMC分泌IFN-a量平均为107．95(48．59~227．33)。CHB患者组显著低于健康对照组，差异具有统计学意义(Z=-2．691，尸=0．007)。CHB患者组显著低于HBV携带者组，差异具有统计学意义(z=-2．206，尸=0．027)。经pol

3、y(I：C)刺激后，三组PBMC分泌IFN．a量差异无统计学意义(，：0．628，P=0．427)。结论较HBV携带者和健康对照组，CHB患者体内PBMC中树突状细胞分泌IFN．a明显下降，而单核细胞分泌IFN-0【量无显著性降低。【关键词】肝炎，乙型。慢性：树突状细胞：干扰素0【；免疫Thefeaturesofperipheralbloodmononuclearcellssecretinglnterferon-alfainpatientswithchronichepatitisBvirusInfectionCHENGZhaojing',ZHOUBaotong,Z

4、HANGLifan，SHIXiaochun，HANYang,zHANGYueqiu．XUHong,LIUXiaoqing．"DepartmentofInfectiousDiseases．PekingUnionMedicalCollegeHospital,ChineseAcademyofMedicalSciences＆PekingUnionMedicalCollege,Beijing100734ChinaCorrespondingauth0rLIUXiaoqing,Email：liuxqpumch@126．com[Abstract]ObjectiveToinvest

5、igatethefeaturesofperipheralbloodmononuclearcell(PBMC)secretinginterferon·alfa(IFN一andevaluatethefunctionofpDCandmonocytetoproduceIFN-dinpatientswithchronichepatitisBvirusinfection．MethodsThefleshbloodsampleswerecollectedfrom16patientswithchronichepatitisB，16agematchedpatientswithHBVc

6、arrierand18agematchedhealthyindividualsascontrolsfromJulyin2012toMarchin2013inPekingUnionMedicalCollegeHospita1．PBMCswereisolated，respectivelyandthenstimulatedinvitrobyCPGoligodeoxynucle0tides(CPGODN)2216orpolyinosinic—polycytidylicacid(polyI：C)asstimulatorsandmeasuredbyenzymelinkedim

7、munosorbentassay(ELISA)forIFN一0【production．ResultswilhCPGODN2216stimulation．thelevelofIFN一8productionwas31．20(7．33-44．04)pg／mlinCHBgroup，109．91(13．74—240．27)pg／mlinHBVcarriergroupand107．95(48．59-227．33)pg／mlinhealthycontrolgroup，respectively．LowerlevelsofIFN-aproductioninCHBgroupwereo

8、bserv