喉鳞状细胞癌中肝细胞生长因子受体的表达及意义-论文.pdf

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1、中国煤炭工业医学杂志2013年11月第16卷第11期·1775·喉鳞状细胞癌中肝细胞生长因子受体的表达及意义戴文成蒋斌韩靓摘要目的研究肝细胞生长因子受体(c—Met)在喉鳞状细胞癌患者组织及血浆中的表达，探讨c—Met在喉鳞状细胞癌发生、发展所起的作用。方法喉鳞状细胞癌及喉乳头状瘤患者进行调查，并且设立正常对照组，应用酶联免疫吸附法检测各组血浆c—Met水平；应用半定量RT—PCR法和免疫组织化学法分别检测各组组织中c—MetmRNA及C—Met蛋白的表达。结果喉鳞状细胞癌患者血浆中的e—Met水平明显高于喉乳头状瘤患者和正常人群(均P<0．05)。喉鳞状细胞癌组织中的C

2、—Met水平明显高于喉乳头状瘤组织和正常组织(均P<0．05)。结论C—Met在喉鳞状细胞癌组织及血浆中呈高表达，可能与喉鳞状细胞癌的发生、发展有关。关键词肝细胞生长因子受体；喉鳞状细胞癌；酶联免疫吸附法；逆转录多聚酶链式反应；免疫组织化学中图分类号R739．65’文献标识码B文章编号1007—9564(2013)11—1775—04D0I编码10．11723／mtgyyx1007—9564201311009TIIEsTUDYFoRTHEEXPRESS10N0FHEPAToCYTEGR0WTHFACT0RRECEPToRITHESERUMANDTISSUESINPATIE

3、NTSWITHLARYNGEALSQUAM0USCELLCARCIN0MASDaiWencheng。JiangBin-HanLiang．DepartmentofHeadandNeckSurgery，NantongTumorHospital，Nantong226361，ChinaAbstractObjectiveTostudytheexpressionofhepatocytegrowthfactorreceptor(c—Met)intheserumandtissuesinpatientswithlaryngealsquamouscellcarcinomas。exploret

4、heroleofC—Metinthetumori—genesisandprogressionofhumanlaryngealsquamouscellcarcinomas．MethodsSixtypatientswithlaryn—gealsquamouscellcarcinomasorfortycasesoflaryngealpapillomawereselectedinauthorshospital-andfortyhealthpersonswereenrolledincontro1group．Theplasmac—Metconcentrationsinthemwasd

5、etec—tedbyenzyme—linkedimmunosorbentassay(ELISA)；theexpressionofe—MetmRNAandproteinweredetectedintissueofthembyusingsemi—quantitativereversetranscription—polymerasechainreactionfRT—PCR)andimmunohistochemistryrespectively．ResultsTheplasmac—Metconcentrationsinpatientswithlaryngealsquamousce

6、llcarcinomaswashigherthanthoseinlaryngealpapillomaandnormalcontrol、group(P

7、gealsquamouscellcarcinomasishigh，c—Metmaybeinvolvedinthetumorigenesisandprogressionoflaryngealsquamouscellcarcinomas．KeywordsHepatocytegrowthfactorreceptor；Laryngealsquamouscellcarcinomas；Enzyme—linkedim—munosorbentassay(ELISA)；Reversetranscription—polymerasechainre