发光二极管红光对实验性高脂血症大鼠血脂的调节作用-论文.pdf

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1、中国康复理论与实践2013年9月第19卷第9期ChinJRehabilTheoryPract，Sep．2013，Vo1．19，No．9824——DOI：10．3969~．issn．1006-9771．2013．09．007·基础研究·发光二极管红光对实验性高脂血症大鼠血脂的调节作用卢建丽，刘翠霞，尹昱，安经克，贾子善I摘要】目的探讨发光二极管(LED)红光照射对高脂血症大鼠血脂的调节作用。方法健康雄性Sprague—Dawley大鼠36只随机分为正常对照组(n=l2)和高脂模型组(n=24)。正常对照组饲喂普通饲料，高脂模型组采用高脂饲料喂养6周。造

2、模成功后，再将高脂模型组随机分为高脂对照组(n=12)和LED治疗组(n=12)。高脂对照组不给予照射，LED治疗组给予LED红光照射，波长(630~15)nm，每天3Omin，连续照射28d。生化法检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL—c)、高密度脂蛋白(HDL．C)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。肝脏组织免疫组化染色观察羟甲基戊二酰辅酶A还原酶(HMG—CR)的表达。结果LED治疗组大鼠血清TC、TG和LDL．C水平较高脂对照组明显减低(P

3、D治疗组血清LPL和HL的活性明显高于高脂对照组(尸

4、bilitation，HebeiGeneralHospital,Sh~iazhuang050051Hebei,ChinaAbstract：ObjectiveToobservetheefectoflightemittingdiode(LED)redlightirradiationonserumlipidinexperimentalhyperlipid—emiarats．Methods36healthymaleSprague—Dawleyratswererandomlydividedintonormalcontrolgrouprn=12)andhyper

5、lipidemicmod—elgroupfn=24)．Thenormalgroupwasfedwithnormaldietwhilethehyperlipidemicmodelgroupwithfat—richforagefor6weeks．Thehy—perlipidemicmodelgroupratswererandomlydividedintothehyperlipidemiccontrolgroup(n12)andLEDtreatmentgroup(n=12)，andthelatteracceptedLEDredlightirradiatio

6、nfor28d．Thelevelsofserumlipidincludingtotalcholesterol(TC)，triglyceride(TG)，low·densi—tylipoprotein(LDL-c)，high—densitylipoprotein(HDL-c)，andtheactivitiesoflipoproteinesterase(LPL)andhepaticlipase(HL)werede—tectedwithbiochemicalassay．Theexpressionof3-hydroxy-3一methylglutarylcoe

7、nzymeAreducase(HMG—CR)ofhepatictissueweremea—suredwithimmunohistochemicalstaining．ResultsComparedwiththehyperlipidemiccontrolgroup，thelevelsofserumTC，TGandLDL—CdecreasedwhiletheserumHDL—CincreasedsignificantlyintheLEDtreatmentgrouprp<0．o1)aftertreatedwithLED．ThelevelsofLPLandHL

8、inserumincreased(尸<0．01)whiletheactivityofHMG-CRdecrea