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《兔肝VX2肿瘤射频消融术后残癌中基质金属蛋白酶9表达-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、.5440·现代生物医学进展wwwashengwuyixue.comProgressinModernBiomedicineVoL14NO28OCT2014doi:10.13241/j.cnki.pmb.2014.28.010兔肝VX2肿瘤射频消融术后残癌中基质金属蛋白酶9表达马艳艳刘颖张绿卓王健琳范秋阳邱天爽。(1大连医科大学附属第二医院体检中心辽宁大连116027;2大连理工大学电子与信息工程学院辽宁大连116024)摘要目的:探讨兔肝脏VX2肿瘤射频消融术(radio—frequencyablation,RFA)后残余肿瘤组织中基质金属蛋白酶9
2、(MMP-9)表达。方法:超声引导下将VX2肿瘤组织块接种于23只新西兰大白兔肝脏中,造模成功后随机分为5组,对照组(A组,n=3),不行RFA治疗;余20只为实验组行RFA治疗,在超声引导下射频针插入肿瘤偏心位置,展开电极致损毁范围最大为肿瘤总体积的2/3.人为造成残存肿瘤组织。根据治疗结束后不同时间点分为4组:0小时组(B组,n=5)、术后1周组(C组,n=5)、术后2周组(D组,n=5)、术后4周组(E组,n=5),行超声检查结束后处死大白兔,取肿瘤组织采取免疫组化法观察残存肿瘤组织中及未治疗肿瘤组中MMP.9的表达情况。结果:RFA术后0小
3、时、l周、2周、4周残存肿瘤组织中MMP一9的表达均较对照组明显降低(P<0.05)。结论:RFA治疗后残存肿瘤细胞中MMP-9水平表达减低。关键词:VX2肿瘤;射频消融;基质金属蛋白酶9;超声检查中图分类号:R735.7文献标识码:A文章编号:1673—6273(2014)28—5440—03TheExpressionOftheMMP.9inRabbitLiverVX2TumOr—remnantr1n10SSUeSat^ter’R⋯adio-1^TequencyA⋯blatl’onMAYan-yan1,L1UYi,ZHANGJing-zhuo~,
4、WANGJian-lif,FANOiu-ysu~,QlUTian-shuange(1Thephysicalexamina6oncentel',The2ridAttachedHospitaltoDalianMedicalUniversity,Dalian,Liaoning,11602ZChina;2DepartmentofElectronicEngineeHng,DalianUniversityofTechnology,Dalian,Liaoning,116024,China)ABSTRACTObjective:Toinvestigatetheexp
5、ressionoftheMMP-9inrabbitliverVX2tumor-remnanttissuesafterradio·-frequencyablationtreatment(RFA).Methods:Ultrasound—guidedtheVX2tumormasswereplantedintotheliverof23NewZealandrabbits,modelsofrabbitVX2livertumorweredividedinto5groupsatrandom,therewere3rabbitsingroupAasthecontrol
6、groupwithnoRFAtreatment;theremaining20rabbitsasexperimentalgroupwiththeRFAtreatment,Ultrasound·guidedtheRfneedlewasinsertedintothelocationoftheliverturnoreccentric,tumor—remnanttissueswereremainedbyopeningtheelectrodetocontrolthemaximumdamagerangeoftotaltumorvolume2/3.20rabbit
7、sweredividedinto4groupsaccordingtotheirdiferenttimeaftertreatment,thatwasafterRFAgroup(Bgroup),oneweekafterRFAgroup(Cgroup),twoweeksafterRFAgroup(Dgroup),fourweeksafterRFAgroup(Egroup),5ratsineachgroup.Therabbitswereexecutedaftertakingultrasonographicalexamination,Immunohistoc
8、hemistrywasusedtodetecttheexpressionstatusofMMP一9inrabbitlive
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