川芎嗪及大鼠CTGF miRNA质粒对高糖刺激肝星状细胞结缔组织生长因子、转化生长因子-beta的影响-论文.pdf

《川芎嗪及大鼠CTGF miRNA质粒对高糖刺激肝星状细胞结缔组织生长因子、转化生长因子-beta的影响-论文.pdf》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、第3l卷第2期生物医学工程学杂志V0I．31NO．22014年4月JournalofBiomedicalEngineeringApril20l4川芎嗪及大鼠CTGFmiRNA质粒对高糖刺激肝星状细胞结缔组织生长因子转化生长因子一beta的影响*、阳宏李军邢旎旎向颖沈艳李孝生1(重庆医科大学附属第二医院消化内科，重庆400010)2(重庆医科大学附属口腔医院牙体牙髓科，重庆401147)摘要：本文旨在研究川芎嗪及大鼠结缔组织生长因子(cTGF)miRNA质粒对高糖刺激大鼠肝星状细胞(HSC)的CTGF和转化生长因子eta(TGF—beta)mRNA表达量、蛋

2、白表达量及I型胶原表达量的作用。首先体外培养大鼠HSC以及稳定表达大鼠CTGFmiRNA和稳定表达阴性质粒的大鼠HSC，高糖及川芎嗪作用后提取总RNA、总蛋白及收集细胞上清液，检测川芎嗪及大鼠CTGFmiRNA质粒对高糖刺激HSC的CTGF、TGF—beta及I型胶原表达量。实验结果表明，高糖导致HSC的CTGFmRNA、CTGF蛋白、TGF—betamRNA、TGF-beta蛋白及I型胶原表达量增加(P

3、GFmiRNA质粒干扰组CTGFmRNA、CTGF蛋白、TGF-betatuRNA、TG卜beta蛋白及I型胶原的表达量较高糖组低(PO．05)，川I芎嗪组I型胶原的表达量明显低于大鼠CTGFmiRNA质粒干扰组(P

4、长因子一beta中图分类号R575．2文献标志码AD0I：lO．7507／1001-5515．20140074EffectofTetramethylpyrazineandRatCTGFmiRNAPlasmidsonConnectiveTissueGrowthFactor，TransformingGrowthFactor。betainHighGlucoseStimulatedHepaticStellateCellsYANGHongLIJunXINGNiniXIANGYingSHENYanLIXiaosheng1(DepartmentofGastroenter

5、ology。theSecondAfJiliatedHospital，ChongqingMedicalUniversity，Chongqing400010，China)2(DepartmentofStomatology，theAffiliatedHospitaloftheStomatology。ChongqingMedicalUniversity，Chongqing401147，China)Abstract：Theaimofthisresearchistoevaluatetheeffectoftetramethylpyrazine(TMP)andconnec

6、tivetissuegrowthfactor(CTGF)miRNAplasmidsontheexpressivelevelsofCTGF，transforminggrowthfactor-beta(TGF—beta)andtypeIcollagenofrathepaticstellatecells(HSC)whicharestimulatedbyhighglucose．TheratHSCswhichweresuccessfullytransfectedratCTGFmiRNAplasmidsandtheratHSOswhichweresuccessfull

7、ytransfect—ednegativeplasmidswereculturedinvitro．AfterstimulusoftheTMPandthehighglucose，theproteinlevelsandgeneexpressivelevelsofCTGF，TGF-betaandtypeIcollagenweretested．TheresultsindicatedthathighglucoseincreasedtheexpressionofCTGFmRNA，CTGFprotein，TGF—betamRNA，TGF—betaproteinandty

8、peIcollagen(P