促肾上腺皮质激素对创伤性休克大鼠急性肺损伤的保护作用及其机制.pdf

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1、·436·实用医药杂志2014年05月第3l卷第O5期PracJMed＆Pharm．Vol31．2014—05No．05基础医学·论著·促肾上腺皮质激素对创伤性休克大鼠急性肺损伤的保护作用及其机制许会彬．杨正友[摘要]目的探讨促。肾上腺皮质激素(ACTH)对创伤性休克(TS)大鼠急性肺损伤的影响及其相关机制。方法Wistar大鼠36只，随机分成假手术组(C组)、模型(1’S)组和ACTH组，每组12只。检测血清肿瘤坏死因子(TNF)一0【和肺组织超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)的含量；计算肺湿干重比(W／D)；光镜

2、下观察肺组织病理变化，并做病理评分。结果与C组比较，TS组与ACTH组肺组织匀浆MDA及血清TNF一水平、肺组织W／D及损伤评分均明显升高(P

3、1：R649．2[文献标识码]AProtectionofandrenocorticotrophichormone(ACTH)onacutelunginjuryassociatedwithtraumaticshockinrats，anditsmechanismXVHui-bin①②，YANGZheng-you．①CollegeofLifeScience，ShandongAgriculturalUniversity，Tai’an，Shandong271000，China．②DepartmentofLaboratory，88thHospitalofP

4、LA，Tai’an，Shandong271000，China[Abstract]ObjectiveToinvestigatetheeffectsofandrenocorticotrophichormone(ACTH)onacutelunginjuryinratswithtraumaticshock(TS)andexploretheunderlyingmechanism．MethodsThe36maleWistarratswereassignedrandomlyinto3groups(n=12，ineach)：shamoperationgroup

5、(C)，TSmodelgroupandACTHpretreatmentgroup．Thearterialbloodsamplesweretakenfordeterminationofserumlevelsoftumornecrosisfactor-Or(TNF-Or)．Thelungtissueswereremovedfordeterminationoflevelsofsuperoxidedismutase(SOD)，malondialdehyde(MDA)andglutathione(GSH)andmicroscopicexamination

6、．ResultsComparedwithgroupC，theserumlevelsofTNF—d，thelunglevelsofMDA，W／Dlungweightratioandpathologicscoresoflungtissueweresignificantlyhigher(P<0．01)，andthelunglevelsofSODandGSHingroupTSandgroupACTHweresignificantlylower(P<0．01)．TheserumlevelofTNF-ot，thelunglevelsofMDA，W／Dlun

7、gweightratioandpathologicscoresoflungtissueweresignificantlylower(P<0．叭orP<0．05)，andthelevelsofSODandGSHweresignificantlyhigher(P<0．01)ingroupACTHthanthoseingroupTS．ConclusionThesupplementationofACTHcanameliorateacutelunginjuryinratswithTS，whichmaycontributetotheinhibitionof

8、oxygenfreeradicalsandinflammatorycytokinesexpression．[Keywords]Traumaticsho