光照对麻醉大鼠海马节律的影响.pdf

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1、现代生物医学进展www~hengwuyixue．comProgressinModernBiomedicineVoL12NO．15MAY2012·2839·光照对麻醉大鼠海马节律的影响水谢康宁罗二平申广浩L△田越郑丽娟汤池闫一力吴小明孙涛刘娟(1第四军医大学生物医学工程学院陕西西安710032；2空军临潼航空医学训练鉴定中心陕西西安710600)摘要目的：研究光照刺激对麻醉大鼠海马节律的影响。方法：对乌拉坦麻醉大鼠，在海马中植入电极，通过神经信号放大器记录场电位。重复对大鼠进行视觉刺激，刺激10s

2、，并给予20s的恢复期。与经典掐尾实验进行对比。结果：乌拉坦麻醉大鼠的掐尾实验能够将海马场电位从”大不规则波”(LIA)变成theta波。LIA到theta的转变通常只需1s，掐尾结束后3s后theta返回LIA。光刺激造成了在5-8s后LIA消失，光刺激撤离后4-6sLIA返回，并没有引发theta。结论：本文结果提示视觉通路与触觉通路有不同的优先级和行为特性。关键阗：大鼠；海马；节律；光刺激中图分类号：Q95．33文献标识码A文章编号：1673—6273(2012)15—2839．03Eff

3、ectsofLightStimu1ationontheHippocampalRhythm．ofAnaesthetizedRats*XIEKang-ning~,LUOEr-pin~,SHENGuang-haoTIANgue；ZHENGLi-juar／,TANGChi1,YANYi-lf,WUXiao-min~,SUNTao1,L1UJuan(1SchoolofBiomedicalEngineeringBiomedicalEngineering,TheFourthMilitaryMedicalUni

4、versity,Xi'an,710032；2LintongAviationMedicalEvaluationandTrainingCentreofChineseAirForce,Xi'an,710600)ABSTRACTObjective：Toinvestigatetheeffectsoflightstimulationonhippocampalrhythmofanaesthetizedrats．Methods：Theratswereanaesthetizedwithurethaneandimp

5、lantedwithelectrodesinthehippocampus，fromwhichneurallocalfieldpotentials(LFPs)wererecorded．Lightstimulationwereappliedtotheratsrepetitivelywith10sdurationandadditional20sdelayforrecovery，whichwascomparedwiththeclassicaltail-pinchexperiment．Results：Th

6、etail—pinchofurethane—anesthetizeratcouldchangetheLFPsinhippocampusfromlargeirregularactivity(L)intothetawave，whichindicatedthatasensoryinputcouldcausetheanimalbeing’active’．ThetransitionofLIAtothetawaveiswithinls．andthatthetawavebacktoLIAisapproxima

7、tely3safterpinch’Sremova1．WefoundthatlightCaneliminateLIAbutnotelicitthemwave，withmuchlongerdelay，5-9sforLIAdisappearingafterlightonsetand4-6sforUA’Sback．Conclusions：Thismaysuggestthatunderurethane-anesthetizedconditionvisualinputislesssensitiveandwi

8、thlowerprioritycomparingwithtactility．Keywords：Rat；Hippocampus；Rhythm；LightstimulationChineseLibraryClassification：Q95-33Documentcode：AArticleI【)：1673—6273r2012)15-2839-03含不同的uA和theta波的组合：轻度麻醉LIA和theta波交替前言出现；中度麻醉场电位主要是LIA，掐尾实验能够引起theta海马属于大脑边缘系统，是哺乳