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时间:2020-04-27
《受体牛血清免疫因子与胚胎附植的关系研究-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中圈奶斗2014·7RI£
2、)R0I)UCT10N&BREI£DING受体牛血清免疫因子与胚胎附植的关系研究于枫’,徐海滨’,张风荣’,侯振中(1.山东畜牧兽医职业学院,潍坊261061;2.东北农业大学,哈尔滨150030)中图分类号:$823.3文献标识码:A文章编号:1004—4264(2014)07—0026—03摘要:本试验通过测定胚胎移植受体牛血清中免疫因子TNF—和IL一6的含量,并将妊娠牛与未妊娠牛测定结果进行对比,研究免疫因子与胚胎附植的关系。结果表明,在胚胎附植过程中,免疫因子TNF—or.含量减少、IL
3、一6增加有利于胚胎附植。关键词:受体牛;胚胎附植;免疫因子;妊娠收稿日期2O13—10-08作者简介于枫(1977一),女,讲师,博士,主要从事动物临床诊断的研究。通讯作者侯振中(1963一),男,教授,博士生导师,主要从事动物产科病研究。测方法的建立U].中国奶牛,2()()6,3:6—8.[19】D.X.Sun,X.HFan,YX1e,etalShortconlnlllnication:【17]YutakaKanae,DaijiEndoh,HajimeNagahata,etalAMethodDistributionofr
4、ecessivegeneticdefectcarriersiI1ChineseforDetectingComplexVertebralMalformationinHolsteinCalvesHolstein[J】JDairySci,20li,94:56955698.usingPolymeraseChainsReaction—PrimerIntroducedRestriction『20】MeydanH,YildizMA,Agerho1mJS.ScreeningforAnalysis[J].JVet1)iagnInvest,20
5、05,17:258262bovinelenkocyteadhesiondeficiency,deficiencyofuridine【18】QinChu,DongxiaoSun,YingYu,etalIdentificationofmonopho叩hatesynthase,complexvertebralmalfornmtion,bovinecomplexvertebralmalformationcarriersinChineseHolstein[J].Jcitrulinaemia,andfactorXIdeficiencyi
6、nHolsteincowsrearedinVetDiagnInvest,20()8,20:228—230Turkey[JJActaVeterinariaScandinavica2010,10:5256.Identi16icati0nofCVMandDUMPSCarriersinChineseHolsteinBullsLIANGRuo-bing,XIEYan,FANXue—hua,ZHANGYi,SUNDong-xiao,ZHANGSheng—li(CollegeofAnimalScienceandTechnology,Chi
7、naAgriculturalUniversity,Beijing100193)Abstract:Complexvertebralmalformation(CVM)anddeficiencyofuridinemonophosphatesynthase(DUMPS)aretwoofHolstein-specificautosomalrecessivedisorders.ThisstudyisaimedtodetectcarriersofbothCVMandDUMPSinChineseHolsteinbulls.Polymeras
8、echainreaction-primerintroducedrestrictionanalysis(PIRA—PCR)andrestrictionfragmentlengthpolymorphism(PCR-RFLP)methodswereusedtodetecttheallelesofCVMandDUMPSamong691Holsteinbullsfrom27bullbreedingstationsparticipatinginthenationalprogenytestbullsprogram.Asaresult,at
9、otalof34CVMcariersandaDUMPScarrierwereidentified.TherecessiveallelefrequencyofCVMandDUMPSis2.46%and0.07%,respectively.Afterpedigreeanalysis,outof
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