条件性Smad4基因敲除鼠模型的建立和鉴定及初步表型-论文.pdf

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1、IntEyeSci，Vo1．14，No．1，Jan．2014www．ies．net．caTel：029-8224517282210956Email：IJO．2000@163．coal·实验论著·条件性Smad4基因敲除鼠模型的建立和鉴定及初步表型刘瑛．林丁作者单位：(410015)中国湖南省长沙市，长沙爱尔眼科医院expressionandtissuespecificityofLe—Crerecombinase作者简介：刘瑛，博士，主治医师，研究方向：眼发育、青光眼。wasalsorevealedb

2、yLacZstainingofRosa；Le—Cre通讯作者：林丁，博士，主任医师，教授，博士研究生导师，研究方doubletransgenicmouse．Accordingto向：青光眼、白内障．dlinoph@126．conImmunohistochemicalstaining．Smad4waswidely收稿Et期：2013—08—02修回日期：2013—12—12expressedinnormalembryoniceyes，mainlyappearinginthecytoplasmatthe

3、earlyembryonicstageandwereEstablishment．identificationandtransferredtonucleuswithgestationdeveloping，whileinmutantembryoniceyes，Smad4wasvoidofexpressioninpreliminaryphenotypeofmousemodelofCre—expressedtissues．ItwasobservedthatSmad4specificSmad4conditio

4、nalgeneknockoutmutantmousecouldsurvJvetheconditionalgeneknockout．ButthosemiceshowedabnormaIappearancesuchasmicrophthalmia，sunkensocket。abnormaIeyelidYingLiu，DingLinopening，andeyelidclosurefailureandperiocularhairIOSS．ChangshaAierEyeHospital，Changsha410

5、015，Hunan·CONCLUSION：Inthisstudy．mousemodelofSmad4Province，ChinaconditionaIknockoutispreciselyestablishedandIackofCorrespondenceto：DingLin．ChangshaAierEyeHospital，expressionofSmad4inmutantmiceisconfirmedbyChangsha410015，HunanProvince，China．dlinoph@126．

6、COBrelatedgeneticandproteicdetection．LackofSmad4Received：2013——08——02Accepted：2013-12-12expressioninspecificoculartissuesofmutantmousecanresultintheabnormalityofeyeandadnexa．whichprovidesareliableanimaImodeJtoinvestigatetheocularAbstractdevelopmentandt

7、herolesofSmad4onit．·AIM：Tomakefurtherphenotypicanalysisby·KEY＼^f0RDS：Smad4；conditionalgeneknockout；Le—Cre；establishingthemousemodelofspecificSmad4reportergene；ocularphenotypeconditionaIgeneknockoutinOCUlartissuebyCre／LoxPsystemofthiskindofmousemode1．Ci

8、tation：LiuY，LinD．Establishment，identificationand·METHODS：MouseofspecificSmad4conditionalpreliminaryphenotypeofmousemodelofspecificSmad4conditionalknockoutinIensectoderm(Le—Cre：Samd4fl／fIoralsogeneknockoutinlensectoderm．C~ojiYankeZazhi(I