病毒性脑炎患者脑脊液肠道病毒和EV71检测与分析-论文.pdf

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1、·784·宁夏医学杂志2014年9月第36卷第9期NingxiaMedJ，Sep．2014，Vol36，No．9Doi：10．13621／j．1001—5949．2014．09．0784·论著·病毒性脑炎患者脑脊液肠道病毒和EV71检测与分析白永生，郭林池，杨安宁，张静，蒋向阳，马静，金立元[摘要】目的探讨肠道病毒和EV71所致脑炎的发病情况及荧光定量PCR诊断病毒性脑炎的意义。方法收集银川市118例临床诊断病毒性脑炎、脑膜炎患者脑脊液和血清，用荧光定量PCR检测肠道病毒和EV71阳性率及病毒拷贝数，用间接酶联免疫吸附试验(ELISA)定

2、性检测血清EV71病毒IgM抗体。结果荧光定量PCR检测肠道病毒阳性病例42例，检测阳性率为35．6％；EV71阳性病例22例，检测阳性率为18．6％，占EV阳性病例的52．3％；ELISA定性检测Ev71病毒IgM抗体，阳性病例30例，检测阳性率25．4％，肠道病毒和EV71病毒性脑炎患者6岁以内幼儿明显多见于其他年龄组。结论肠道病毒是本地区病毒性脑炎、脑膜炎患者最常见的病原体，EV71可能是本地区肠道病毒性脑炎中最多见的一种，肠道病毒和EV71感染所致脑炎都以6以内幼儿为主；荧光定量PCR对EV71检测特异性高于ELISA。[关键词]

3、肠道病毒7l型；肠道病毒；病毒性脑炎或脑膜炎；荧光定量PCR【中图分类号】R725[文献标识码】AAnalysisofenterovirusesandenterovirus71incerebrospinalfluidinpafien~withviralencephalitisBAIYongsheng，GUOLinchi，YANGAnning3ZHANGJing4JIANGXiangyang’，MAJing2，JINLiyuan．1．DepartmentofNeurolo·，，gY，NingxiaPeopleHospital，Yinchua

4、n750002，China；2．DepartmentofComprehensiveInternalMedicine，NingxiaPeople"sHospital，n-chuan750002，China；3．NingxiaMedwalUniversity，Yinchuan750004，China；4．~inwalLaboratory，NingxiaPeople~Hospital，Yinchuan750002，China[Abstract]ObjectiveToinvestigatetheincidenceofviralencephalit

5、is／meningitiscausedbyenteroviruses(EV)andEV71andthevalueofFQ—PCRindiagnosisofencephalitis．MethodsCerebrospinalfluidandseI'umwerecollectedfrom118patientswithclinicaldiagnosisofviralencephalitis／meningitisinYinchuanhospitals．ThepositiverateofenteroviruseswasdetectedbyFQ—PCR

6、；thepositiverateofEV71wasdetectedbyFQ—PCR，andtheIgMantibodyagainstEV71wasexaminedbyusingenzyme—linkedimmunosor-bentassay(ELISA)．ResultsByusingFQ—PCRforenterovirusesinCSF，42outof118patientswerepositiveforenteroviruses(41．9％)，and22outof118patientswerepositiveforEV71enterovi

7、rus(18．6％)．Amongthepatientspositiveforenteroviruses，52．3％(22／42)patientswerepositiveforEV71，while30outof118patientswerepositiveforIgMantibodyagainstEv71(25．4％)whenexaminedusingELISA．Inaddition，theviralencephalitis／menintispatientscausedbyenterovirusesandEV71weremorecommon

8、inchildrenlessthan6yearsold．ConclusionEnterovirusesarethemainpathogensresponsibleforviralencepha