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1、联组检测梅毒特异性及非特异性抗体效果观察.1%、94.6%、75.0%和96.4%;78例一期梅毒四种方法阳性率分别为97.4%、97.4%、96.2%和100.0%;102例二期梅毒四种方法阳性检出率均为100%;66例三期梅毒四种方法阳性率分别为100.0%、93.9%、66.7%和100.0%。联组检测试验组中ELISA法、胶体金法、TRUST法三种方法各自对潜伏期、一期和三期梅毒病例阳性检出率虽与对照组TPPA法稍有差别,但联组判别结果后56例潜伏期梅毒病例实际阳性54例,与对照组阳性率相等;78例一期、102
2、例二期和66例三期梅毒,联组试验结果和对照组阳性检出率均为100%,二者对此三期梅毒检出结果比较差异无统计学意义(P>0.05)o结论联组运用特异性抗体检测试验ELISA法、胶体金法和非特异性抗体检测试验TRUST法检测不同期梅毒病例检出效果较好[关键词]特异性;抗体;联合;检测;梅毒[中图分类号]R759.1[文献标识码]A[文章编号]2095-0616(2017)05-19-04[Abstract]ObjectiveToobservetheeffectofspecificantibodytestELISAmetho
3、d,colloidalgoldmethodandnon-specificantibodytestTRUSTmethodinjointdetectionofdiagnosisofsyphilis・MethodsThejointofELISAmethod,colloidalgoldmethodandTRUSTmethodwasusedtodetectanddiagnosethe302casesofsyphilis.InwhichtwoormoreresuItswerefoundforpositivegroup.Atthes
4、ametime,allsamplesweredetectedbyTPPAmethod.Thepositivedetectionrateoffourmethodsforeachstageofsyphiliswascalculated.Thetestresultswerecomparedbetweenthetestgroupandthecontrolgroup・ResuIts56casesoflatentsyphiliscasesELISA,colloidalgold,TRUSTandTPPApositivedetecti
5、onrateswere91.1%,94.6%,75%and96.4%,respectively.78casesofprimarysyphilisoffourmethodspositiverateswere97.4%,97.4%,96.2%and100%,respectively.102casesofsecondarysyphilispositiverateofthefourmethodswere100%.66casesofthethreestagesyphilispositiveratesofthefourmethod
6、swere100%,93.9%,66.7%and100%,respectively.TherewasslightlydifferentonrelevanceratiobetweenELISAmethod,colloidalgoldmethodandTRUSTjointtestgroupinthreemethodsoflatency,oneandthreestageandTPPAofcontrolgroup・ButtheresuItsof56casesoflatentsyphiliscasesgroupafterthea
7、ctual54casesofpositivecontrolgroup,andthepositiverateswereequa1.Forthe78casesofprimarysyphilis,102casesofsecondarysyphilisand66casesofthreestagesyphilis,thepositivedetectionratesofjointtestresuItsandcontrolgroupwere100%.Therewasnostatisticaldiffereneebetweenthet
8、wogroupsinthedetectionofsyphilisinthethreestage(P>0.05).ConclusionTheeffectofspecificantibodytestELISAmethod,colloidalgoldmethodandnon-specificantibodytestTRUSTmethod