大豆苷元对异丙肾上腺素所致心肌肥厚的保护作用-论文.pdf

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1、：!：旦!△』QQ垦丛垦垦曼!卫QNDDISASTERMEDICINEOctober2012．Vo1．7．N。10·论著·大豆苷元对异丙肾上腺素所致心肌肥厚的保护作用钟星明李良东黄志华曾靖【摘要】目的建立异丙肾上腺素(Iso)诱导的大鼠心肌肥厚模型，观察大豆苷元(DD)对大鼠心肌肥厚时抗氧化功能的影响。方法健康雄性sD大鼠随机分为5组：对照组、心肌肥厚模型组、溶剂对照组、低剂量0．1mo1．kg-及高剂量0．3mo1．kDD组。各组分别背部皮下注射同体积Ns或异丙肾上腺素lmg．kg--，每日一次，连续1Od，建立心肌肥厚模型。连续皮下给药14d。通过测定全心重量

2、参数、左心室重量指数、心肌组织羟脯氨酸含量、血清MDA含量和SOD活性来判断DD对心肌肥厚的保护作用。结果模型组和溶剂对照组的全心重量参数、左心室重量指数及心肌组织羟脯氨酸含量明显高于对照组(P

3、对异丙肾上腺素诱导的大鼠心肌肥厚具有保护作用，提高大鼠的抗氧化功能是DD抗心肌肥厚的机制之一。【关键词】大豆苷元；异丙肾上腺素；心肌肥厚；抗氧化作用myocardialhyrzophyinratsZHONGXing-ming,LILiang-dong,HUANGZhi-hua．ZENGJing．TheFirstA插dhospitalofGannanMedicalUniversity,Ganzhou，Jiangxi341000,ChinaCorrespondingauthor：ZENGJing；Email：qixinu@163．com[Abstract]Object

4、iveToexploredaidzein’S(DD)anti-oxidationeffectonrat’Smyocardialhypertrophyinducedbyisopropylarterenol(Iso)．MethodsTherat’Smyocardialhypertrophymodelwasformedthroughhypodermicinjectionwhichextended10daysatbackskinwithIso(1mg．kg-1．d)，andwasadministratedwithDD(O．1or0．3umo]．kg-1．d)，DMSOor

5、NSinintragastricwayfromthesecondday，andextendto14days．Afterthelastadministrat!on，theratwasputinabsolutedietstatusfor12hoursanditsbloodandheartwastakenunderanesthesia．Themyocardialhypertrophyindexexpressedonheartweight／bodyweight(HW／BW)andleftventricleweight／bodyweight(LVW／B．Theconcent

6、rationofMDAandhydroxyprolineandtheactivitiesofSODandGSH-PXinserumwereanalyzed．ResultsItdemonstratedthatHW／BWandLVW／BWofIsogroupweremuchbiggerthanthecontrolgroup，andtheconcentrationofMDAinserumandhydroxyprolineinmyocardiumwerealsoenhanced．ComparedwithIsogroup，DDgroupsreflecteddecreases

7、intheindex，hydroxyprolinandMDAlevel，andincreasesintheactivitiesofSODandGSH-Px．ConclusionDDreflectedaprotectiveeffectO13Iso—inducedmyocardialhypertrophyinrat．Itmayberelatedtoreducingtheoxidativestressandimprovingantioxidantcapacity．[Keywords]Daidzein；Isopropylarterenol；Myocardialhypert

8、rophy