大豆苷元对结扎大鼠冠状动脉所致损伤的保护作用.doc

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1、大豆苷元对结扎大藏冠状动脉所致损伤的保护作用作者：叶和杨，胡志苹，周莉，黄志华，曾靖【关键词】大豆ProtectiveeffectofDa.idzEinonmyocardialischemiainjuryinrats【Abstract】AIM：ToobservetheprotectiveeffectofDaidzEInonratswhosebranchofleftanteriordescendingcoronaryarterieswereligated.METHODS：Ratsischemia.injurymode

2、lwasestablishedbyligatingthebranchofleftanteriordescendingcoronaryarteryforadurativecourseof120min.Thirtytwomaleratswererandomlydividedinto4groups：shamoperationgroup,modelgroup，highdose(mg/kg)DaidzeingroupandlowdoseDaidzeingroup.ThetwoDaidzeingroupswereinjecte

3、dwithDaidzeinoflmL/10OgthroughsublinguaIveinlOminbeforeischemiainjuryandsalinewasgiveninshamoperationgroupandmodelgroup.TheleadIIECGwasrecordedbeforemyocardialischemiainjury,15minafterischemiaand30,60,90andl20minafterinjectionwithDaidzein.TheZSTofleadIIECGatea

4、chmomentmentionedabovewasanalyzedandtheJpointandTwaardialischemiamodeluatetheprotectiveheactivityofcreatinephosphokinase(CKgenase(LDH)inserumndialdehyde(MDA)anse(SOD)inmyocardiadtoevaluatetheprotzein.ThemyocardialuredbyTTCstainingofcardiacmusclecellreofECGinac

5、utemyoclwereobservedtoevaeffectofDaidzein.T)andlactatedehydro,thecontentsofmalodsuperoxidedismutaltissuewereobserveectiveeffectofDaidinfarctsizewasmeassinanother24rats(dividedinto3groups：nmg/kggroup,Daidzeyocardialischemiaiamemethods.RESULTSmodelgroup,Daidzeii

6、nmg/kggroup)withmnjuryinducedbythes:Comparedwiththoseinmodelgroup,theaalinfarctiondeclinreaofacutemyocardied,thevalueofZSTreducedandthemaximumvaluesofJpointandTwareofECGdeclinedinthe2Daidzeingroups.TheactivityofLDHandCKinthe2Daidzeingroupswaslowerthanthatinthe

7、modelgroup.ThecontentofSODwassignificantlyhigherandMDAwasmarkedlylowerintheDaidzeingroupsthanthoseinthemodelgroup.A1lthechangesmentionedaboveshowedsomedosedependenteffect.CONCLUSION：Daidzeinhasobviousprotectiveeffectonmyocardiumagainstischemiainjurycausedbylig

8、atingthebranchof1eftanteriordescendingcoronaryartery.【Keywords】Daidzein；myocardialischemia；coronaryarteryligation；serumenzyme；freeradicals【摘要】目的：观察大豆苷元对结扎大鼠左冠状动脉前降支损伤的保护作用.方法：取32只雄