欢迎来到天天文库
浏览记录
ID:53270173
大小:580.60 KB
页数:4页
时间:2020-04-17
《月光花豆乙醇提取物对急性肺损伤大鼠的保护作用-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、医药导报2014年5月第33卷第5期·569·月光花豆乙醇提取物对急性肺损伤大鼠的保护作用唐秀能,韦红棉,黄仁彬(1.广西医科大学药理学教研室,南宁530021;2.广西中医药大学附属瑞康医院,南宁530011)摘要目的探讨月光花豆乙醇提取物(CABE)对急性肺损伤(ALI)大鼠的保护作用。方法大鼠尾静脉注射脂多糖5mg·kg复制ALI模型,免疫组化法测定大鼠肺组织中核因子一KBp65(NF—KBp65)的表达,酶联免疫吸附(ELISA)法测定肺组织白细胞介素一1p(IL一1B)的含量,同时观察肺组织病理形态、肺组织的湿干质量比例(W/D)和肺组织髓过氧化物酶(MPO)的活性。结果模型大鼠和C
2、ABE治疗大鼠肺组织W/D分别为5.05±0.24,4.35±0.21;IL一1B含量分别为(320.60+19.73),(294.42+-13.95)Pg·mL~;MPO活性分别为(1.31±0.18),(1.04+0.18)U·g~;与模型大鼠比较,CABE治疗大鼠肺组织损伤减轻,IL一113含量减少,肺组织W/D和MPO活性降低,NF—KBp65表达降低(P<0.01)。结论CABE对脂多糖诱导的ALI大鼠具有明显的保护作用,干扰NF—KB途径和抑制IL一1B的生成可能是其作用机制之一。关键词月光花豆乙醇提取物;损伤,肺,急性;核因子;白细胞介素中图分类号R282.71;R285.5文献
3、标识码A文章编号1004-0781(2014)O5—0569—04ProtectiveEfectsofEthanolExtractsofCa/onycaonACClileatumBeansonRatswithAcuteLungInjuryTANGXiu-neng’,WEIHong-mian,HUANGRen-bin(1.DepartmentofPharmacology,GuangxiMedicalUniversity,Nanning530021,China;2.RuikangHospitalAffiliatedtoGuangxiUnivemityofChineseMedicine,Nannin
4、g530011.China)ABSTRACTObjectiveToinvestigatetheprotectiveeffectsofethanolextractofcalonyctionacculeatumbeans(CABE)onratswithacutelunginjury(ALI).MethodsTheALImodelwasinducedviaintravenousinjectionwith5mg·kglipopolysaccharide(LPS).Theexpressionofnuclearfactor—KBp65(NF—KBp65)wasdetermindbyimmunohistoc
5、hemistry(IHC),andthecontentofinterleukin.18(IL一18)inthelungtissuewasdetectedbyenzyme—linkedimmunosorbentassay(ELISA).Meanwhile,lungtissuehistopathology,thewet—dryweightratiooflungandtheactivityofmyeloperoxidase(MPO)inthelungtissueswereobserved.ResultsThewet—dryweightratiooflungofthemodelgroupandCABE
6、treatmentgroupwas5.05±0.24,4.35±0.21,respectively;thecontentofIL一1Bwas(320.60-+19.73),(294.42+13.95)Pg·mL~,respectively;theMPOactivitywas(1.31-+0.18),(1.04_+0.18)U·g~,respectively.Comparedwiththemodelgroup,CABElessenedthelunginjury,decreasedIL一1Blevel,reducedthelungdry—wetweightratioandactivityofMPO
7、,anddown-regulatedtheexpressionofNF—KB/I)65(P<0.05).ConclusionCABEhasremarkedlyprotectiveeffectonALIinducedbyLPSinrats.OneofthemechanismsmayberelatedtoreducingthesecretionofIL一1Bandinterferingwiththea
此文档下载收益归作者所有