玉米-大豆轮作及氮肥施用对土壤细菌群落结构的影响-论文.pdf

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1、作物学报ACTAAGRONOMICAS~ICA2013，39(11)：2016-2022http：／／zwxb．chinacrops．org／ISSN0496—3490；CODENTSHPA9E—mail：xbzw@chinajourna1．net．caDoI：10．3724／SP．J．1006．2013．02016玉米．大豆轮作及氮肥施用对土壤细菌群落结构的影响周岚，2，杨永，王占海陈阜曾昭海，中国农业大学农学与生物技术学院，北京100193；吉林农业科技学院，吉林吉林132101；呼伦贝尔市农业科学研究所，内蒙古扎兰屯162650摘要：在大豆开花期分

2、别对3个施氮水平下(0、50和100kghm-2)大豆连作(大豆一大豆．大豆)、玉米．大豆轮作I(大豆．玉米．大豆)及玉米一大豆轮作II(玉米．玉米．大豆)，应用PCRDGGE技术研究玉米．大豆轮作及施氮对土壤细菌群落结构变化的影响。结果表明，随着施氮水平的提高，3种种植方式土壤中细菌群落多样性、丰富度均呈减少趋势。高氮处理(100kghm-2)明显降低了大豆连作、玉米一大豆轮作II根际土壤细菌群落多样性及丰富度，玉米一大豆轮作I根际土壤细菌群落多样性及丰富度略有降低。玉米一大豆轮作1种植方式可减轻氮肥对其根际细菌群落多样性和丰富度的影响，但施氮明显改变

3、了其细菌群落结构。玉米一大豆轮作II中大豆根际土壤细菌群落结构较为稳定，受氮肥影响较小。在3种种植方式的土壤中，分布着酸杆菌门、变形菌门及厚壁菌门细菌，其中前两门菌群占主导地位。关键词：轮作；氮肥；大豆；玉米；DGGE；细菌群落InfluenceofMaize-SoybeanRotationandNFertilizeronBacterialCommunityCompositionZHOULan，，，YANGYong，，WANGZhan．Hai，CHENFu，andZENGZhao—Hai'CollegeofAgricultureandBioteclmol

4、ogy，Beijing100193，China；。JilinAgriculturalScienceandTechnologyCollege，Jilin132101，China；HulunbeierAgriculturalInstitute，Zhalantun162650，ChinaAbstract：Insoybeanfloweringperiod，tookrhizospheresoilsamplesfromthreetreatmentsincludingcontinuouscroppingsoy—bean(soybean—soybean—soybean)

5、，maize—soybeanrotationI(soybean—maize—soybean)andmaize—soybeanrotationII(maize—maize—soybean)．Eachtreatmenthadthreelevelsofnitrogenapplicationincluding0，50，and100kgha～．SoilmicrobialcOInlTIU—nitychangesundermaize-soybeanrotationsystemandvariousnitrogenapplicationswereinvestigatedb

6、ythetechniquesofdena-turinggradientgelelectrophoresis(DGGE)analysis．TheresultsshowedthatthebiologicaldiversityandabundanceofthreekindsofsoilmicrobialcommunityhadadeclineⅡ_endalongwiththeincreaseofnitrogenapplicationlevels．Underhighnitrogenappli—cationlevel(100kgha一)，asignificantd

7、ecreaseindiversityandabundanceofrhizospheresoilmicrobialcommunitieswasob．servedinbothcontinuouscroppingsoybeansystemandmaize—soybeanrotationIIsystem．Andinmaize—soybeanrotationIsystem，therewasonlyaslightlydecreaseindiversityandabundanceofrhizospheresoilmicrobialcommunities．Therefo

8、re，1-yearmaizeinrotationwithsoybean(soyb