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1、第28卷第6期生态学报Vo.l28,No.62008年6月ACTAECOLOGICASINICAJun.,2008不同肥力条件下的桑树根际微生物种群分析1,21111,*吴凡,李传荣,崔萍,夏尚远,刘训理(1.山东农业大学林学院,泰安271018;2.湖北省农业科学院经济作物研究所,武汉430064)摘要:通过平板梯度稀释培养,测定了不同肥力土壤桑树根际细菌、真菌、放线菌和三类促生细菌的数量,并采用BOX-PCR技术,对根际细菌进行了聚类分析。主要结果为:肥沃土壤根际细菌和放线菌的数量均高于贫瘠土壤,而真菌数量低于贫瘠土壤;相同肥力条件下,三类促生细菌中溶磷细菌的数
2、量最多,其次是硅酸盐细菌,固氮细菌的数量最少。根际细菌的BOX-PCR聚类分析图显示,肥沃土壤细菌分离株的DNA同源性高于贫瘠土壤,两种土壤中,促生细菌分离株的遗传进化距离比较接近。在细菌BOX-PCR图谱相异百分数为0.2的水平上,肥沃和贫瘠土壤根际细菌分别分为71个和33个聚类群,根际促生细菌分别分为33个和28个聚类群。土壤肥力对根际细菌的种类和数量都有影响,肥沃土壤根际细菌和促生细菌的数量、种类均高于贫瘠土壤。关键词:桑树;根际微生物;聚类分析;多样性文章编号:1000-0933(2008)06-2674-08中图分类号:Q143,Q145,Q938文献标识
3、码:APopulationanalysisofmulberryrhizospheremicrobesindifferentsoilfertilities1,21111,*WUFan,LIChuan-Rong,CUIPing,XIAShang-Yuan,LIUXun-Li1ForestryCollege,ShandongAgriculturalUniversity,Taian271018,China2IndustrialCropsInstituteofHubeiAcademyofAgriculturalSciences,Wuhan430064,ChinaActaEco
4、logicaSinica,2008,28(6):2674~2681.Abstract:Therhizospheresoilsweresampledbythree-point-samplingmethodfrommulberryfieldsofdifferentfertilitylevelsinTaian,ShandongProvince.ThemulberryvarietyplantedwasHusang32andof8yearscwoodage.Rhizospherebacteria,fungiandactinomycesinbothfertileandinfer
5、tilesoilswereisolatedandcultivatedbyplatespreparedwithselectivemedia,andthepopulationsofeachkindofmicrobeswereexaminedbyCFUcounting.Rhizospherebacteriainterestedincludedtotalbacteria,azotobacter,phosphate-dissolvingbacteriaandpotassium-dissolvingbacteria.BOXpolymerasechainreaction(BOX-
6、PCR)wasusedtofingerprintdifferentrhizospherebacteria.Themainresultswereasfollows:therhizospherebacteria,azotobacter,phosphate-dissolvingbacteria,potassium-dissolvingbacteriaandactinomycesinfertilesoilsweremoreabundantthanthoseininfertilesoils,whereasthefungiinfertilesoilswerelessthanth
7、oseininfertilesoils.Insoilsofthesamefertilityleve,lphosphate-dissolvingbacteriaweremostabundant,followedbypotassium-dissolvingbacteria,whileazotobacterrankedtheleast.BasedontheBOX-PCRfingerprintsclusteranalysisofrhizospherebacteria,theDNAhomologyofrhizospherebacterialisolatesfromfert
