简明培养基配方.doc

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1、一.White培养基，White(1963)1.无机盐类Ca(N0j2•41LO287mg/LKNO380mg/LKC165mg/LNaH2P04•H2019.1mg/LMgS04•7H20738mg/LNa2S04：•10IL0453mg/LMnSO4•4H206.6mg/LH3B031.5mg/LZnSO4•7H202.7mg/LKI0.75mg/L2.有机物甘氨酸3.0mg/L烟酸0.5mg/L维生素B60.1mg/L维生素Bl0.1mg/L柠檬酸2.0mg/L蔗糖20g/LpH5.7二、LS培养基RM~64[Linsmair&SKoog(1964))]1.无机盐NH

2、iN031650mg/LKN031900mg/LCaCl2•2H20440mg/LMgS04•7H20370mg/LKII2P04170mg/LNa2一*EDTA37.3mg/LFeS04-7H2027.8mg/LH3B036.2mg/LMnS04•4H2022.3mg/LZ11SO4•7IL08.6mg/LKI0.83mg/LNa2MoO•2H200.25mg/LCuS04•5H200.025mg/LCoCl2•6H200.025mg/L2.有机物维生素Bl0.4mg/L肌醇10.Omg/L蔗糖30g/LpH5.6*FeS04-7H205.57g,Na2—EDTA7.45

3、g先溶于1升蒸憾水里，然后按每升培养基含5mg的比例加入培养基屮。三・HB培养基Holley&Baker(1963)1.无机盐(以1/2浓度的Knop液为基础)Ca(N03)2.4H201000mg/LKNO3125mg/LMgS04•7H20125mg/LKII2P04125mg/LBerthelot，s液0.5ml/LBerthelot's液成分组成:MnS04•4H2020g/LKI0.5g/LNiCl2•6II200.05g/LCoCl2•6H200.05g/LZnS04•7H200.10g/LCuS04•5H200.05g/LBeS04•4H200.10g/LH3

4、B030.05g/L浓硫酸1ml2.有机物维生素Bl理学1mg/L腺嚓吟8mg/LNAA2mg/L蔗糖40g/L四・MS培养基RM62(Murashige&Skoog(1962))1•无机盐类同LS培养基2.有机物五.花肥培养基Kano(1963)(Kyotosolution)花肥3g/LKH2P04250mg/LCa(X03)2.4H20lOOOmg/LMgSOr7H20250mg/L(NH4)2S042mg/L蔗糖20g/LpH5.0六.KC培养杲Knudson0(1964)1・无机盐KH2PO4250mg/LCa(X03)2.4H20lOOOmg/L(NH4)2S04

5、500mg/LMgSOi-7H20250mg/LFeS04•7H2025mg/LMnS04•4H207.5mg/L2.有机物蔗糖20g/LpH5.0〜5.2七.Kassanis培养基(1957)1・无机盐八.木本植物用培养基(WPM,WoodyPlantmedium)1•无机盐九.Nitsch&Nitsch(1969)培养基1.无机盐Ca(N03)2.4H20500mg/LKNO3125mg/LMgSO4•7H20125mg/LKH2P04125mg/LBerthelot，s液10滴2.有机物酪蛋白1mg/L半胱氨酸10mg/L腺嚓吟5mg/L烟酸1mg/L维生素B61mg

6、/L泛酸钙10mg/L肌醇0.1mg/L维生素H(生物素)0.01mg/L蔗糖20g/LNH4N03400mg/Ca(N03)2.4H20556mg/LK2S04990mg/LCaCl2•2H2096mg/LKH2P04170mg/LNa2Mo04•2H200.25mg/LMgSOr7H20370mg/LMnS04•H2022.4mg/LZnS04-7H208.6mg/LCuS04•5H200.25mg/LFeS04•7H2027.8mg/LNa2-EDTA37.3mg/L2.有机物肌醇100mg/L维生素Bl1.Omg/L烟酸0.5mg/L维生素B60.5mg/L甘氨酸2

7、.0mg/L蔗糖20g/L琼脂6g/LpH5.2KNO3950mg/LNH4N03720mg/LKII2P0,68mg/LCaCL•2H20166mg/LMgSO4•7H20185mg/LFeSO4•7H2027.8mg/LNa2-EDTA37.3mg/LMnSOi•4H2025mg/LH3B0310mg/LZnSO4•7H201Omg/LCuS04•5H200.025mg/LNa2Mo04•2H200.25mg/L2.有机物维生素H(生物素)0.05mg/L肌醇100mg/L维生素Bl0.5mg/L烟酸5mg/L