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1、2010年23卷5期西南农业学报Vol23No5SouthwestChinaJournalofAgriculturalSciences1533文章编号:1001-4829(2010)05-1533-07西贡蕉枯萎病生防木霉菌株gz2的鉴定及生物学特性研究12*3141柯仿钢,黄思良,付岗,汪茜,张欣,黎起秦(1.广西大学农学院,广西南宁530005;2.南阳师范学院,河南南阳473061;3.广西农业科学院微生物研究所,广西南宁530007;4.中国热带农业科学院环境与植物保护研究所,海南儋州571737)摘要:拮抗西贡
2、蕉枯萎病菌的木霉菌株gz2是一株具有生防潜力的拮抗菌,依据其形态特征和ITS序列将其鉴定为哈茨木霉(TrichodermaharzianumRifai)。gz2菌株的生物学特性测定结果表明,该菌株最适宜在马铃薯蔗糖琼脂(PSA)培养基上生长;蔗糖、酵母膏分别为最佳碳氮源;菌丝最适生长温度范围为28~30;最适pH5.0~6.0;不同光照条件对该菌产孢影响明显。对西贡蕉枯萎病的盆栽防治试验结果表明,gz2菌株对西贡蕉枯萎病具有一定的防效,其中每隔7d施1次gz2固体菌剂处理的防治效果最好,在伤根接种60d后,平均防效达到74.4%。关键词:西贡蕉;枯萎病;木霉菌株gz2;生
3、物学特性;防治效果中图分类号:S688.1文献标识码:AIdentificationandBiologicalCharacteristicsofTrichodermaStraingz2,ABiocontrolAgentagainstSaigonBananaFusariumWiltKEFanggang1,HUANGSiliang2*,FUGang3,WANGQian1,ZHANGXin4,LIQiqin1(1.AgriculturalCollege,GuangxiUniversity,NanningGuangxi530005,China;2.NanyangNormalUn
4、iversity,NanyangHenan473061,China;3.MicrobiologyResearchInstitute,GuangxiAcademyofAgriculturalSciences,NanningGuangxi530007,China;4.EnvironmentandPlantProtectionInstitute,ChineseAcademyofTropicalAgriculturalSciences,DanzhouHainan571737,China)Abstract:Trichodermastraingz2isapotentialbiocontrola
5、gentagainstFusariumwiltofSaigonbanana.ThestrainwasidentifiedasTrichodermaharzianumRifaibasedonitsmorphologicalcharacteristicsandsequenceanalysisoftheinternaltranscribedspacerregionoftheribosomalDNA(ITS).Thebiologicalcharacteristicsofstraingz2werestudied.Theresultsshowedthatthegrowthrateandco
6、nidianumberofthebiocontrolstrainwerefasterandmoreinPSAmediumthanthoseinothermedia.Sucroseandyeastextractweretheoptimumcarbonsourceandnitrogensourceforvegetativegrowthandsporulation,respectively.Theoptimumtemperatureformyceliumgrowthandconidialgerminationwas28-30,andtheoptimumpHvaluewas5.0-6.0.
7、Lightconditionshadobviousinfluencesonsporulationsofthebiocontrolstrain.Theresultsofpotexperimentofstraingz2againstSaigonbananaFusariumwiltindicatedthatthecontroleffectofstraingz2againstSaigonbananaFusariumwiltwasbestwhensolidst
