昆虫病原真菌与硅藻土混用对四纹豆象的毒力效果-论文.pdf

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1、昆虫学报ActaEntomologicaSinica，November2012，55(11)：1282—1288ISSN0454-6296Eofentomopathogenicfungiincombinationwithaceousearthagainstllosobruchusmacula：tus{a莹．鼋(Coleoptera：Bruchidae)mNedaNABAEI，，AllMEHRVAR，MoosaSABERMohammadBAGHERI，(1．DepartmentofPlantProtection，Facul

2、tyofAgriculture，UniversityofMaragheh，55181—83111，Maragheh，Iran；2DepartmentofPlantProtection，FacultyofAgriculture，AzarbaijanShahidMadaniUniversity，53751-71379，Tabriz，Iran)Abstract：Experimentswereconductedtoenhancethevirulenceoftwoisolateso±ent0mopath0gen1ctungi，IR

3、AN一187CofBeauveriabassiana(Balsamo)Vuillemin，andA一396isolateofMetarhiziumanisopliae(Metschnikow)Sorokin，bycombinationwithInsectoSec@formulationofdiatomaceousearth(DE)againsttheaduhsofCallosobruchusmaculatusF．underlaboratoryconditions．Theresultsdemonstratedthatthe

4、combinationsbetweendiatomaceousearthandthefungalisolatesat1ethalandsub—lethaldosesachievedhighmortalitvratesintheadultpopulations；moreover，thetreatmentsimprovedLT50valuesofthefungi．ThelowestvalueofLT(122．2h)wasachievedbyLC50(B．bassiana，IRAN—l87C)+LC50(DE)treatmen

5、twhichwasonparwiththat0fLC(M．anisopliae，A．396)+LC50(DE)combination(128．2h)basedontheir95％riduciallimitvalues．resultinginbettereficacyofeachfungalisolateincombinationwithdiatomaceousearthagainstC．macuZ0adults．Also，effectsofthethreetemperatures(22，27，and30℃)andtwor

6、elativehumiditylevels(40％and55％)ontheactivityofdiatomaceousearthwhenappliedagainstC．maculatusaduhsshowedthatthe1eastvalueofLTn(110．1lh)wasattainedin30℃andRHof4O％，suggestinghighactivitvofdiatomaceousearthinhightemperaturesandlowhumidityconditions．Keywords：Callosob

7、ruchusmaculatus；Beauveriabassiana；Metarhiziumanisopliae；diatomaceousearth；virulence；medianlethalconcentration(LC0)；medianlethaltime(LT50)；mortalityozonelayer．Thefutureuseofphosphinealsocould1INTRoDUCTIoNbethreatenedbythedevelopmentofresistantstrainsofpests(Moravv

8、ejandAbbar，2008)．Therefore，Beauveriabassiana(Balsamo)VuilleminandinsecticideresistanceandtheconsequentlossesofMetarhiziumanisopliae(Metschnikow)Sorokinaref0odd