超声靶向转染缺氧诱导因子干扰基因治疗大鼠肝癌的研究

《超声靶向转染缺氧诱导因子干扰基因治疗大鼠肝癌的研究》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、第43卷第1期第12页华中科技大学学报(医学版)Vo1．43No．1P．122014年2月ActaMedUnivSciTeehnolHuazhongFeb．2O14超声靶向转染缺氧诱导因子干扰基因治疗大鼠肝癌的研究*丁尚伟，黄晓宇，姚梦云，张培歌，武或，于艾嘉，吕清，张丽，张艳容△华中科技大学同济医学院附属协和医院超声影像科，武汉430022摘要：目的利用超声靶向转染缺氧诱导因子干扰基因(HIF-lashRNA)，观察其对肝癌的治疗效果。方法构建Wistar大鼠肝癌种植瘤模型。随机将大鼠分为两组：假手术组，开腹后不

2、进行超声辐照治疗；超声靶向基因转染(UT—MD)组，开腹后将基因与微泡混合后经尾静脉匀速注入，同时进行超声靶向辐照。分别于治疗后第3天、第7天、第14天行超声造影检测肿瘤大小的变化(计算公式为体积一长×宽×高×／6)，qPCR检测HIF一1amRNA的变化，Western和免疫组化检测肿瘤细胞内HIF-la蛋白的表达。结果治疗前两组的肿瘤大小差异无统计学意义。治疗后第3天两组肿瘤均有一定的增大，两组肿瘤大小差异有统计学意义(P

3、统计学意义(P

4、014．01．003TreatmentofLiverCancerofRatbyUTMD-—mediatedHIF--lotshRNATransfectionDingShangwei，HuangXiaoyu，YaoMengyunetalDepartmentofUltrasound，UnionHospital，TongjiMedicalCollege，HuazhongUniversityofScienceandTechnology，Wuhan430022，ChinaAbstraetObjectiveToevaluate

5、thetherapeuticeffectofhypoxia—induciblefactor-1a(HIF一1a)shRNAdeliveredbyultra—soundonratlivercancer．MethodsTransplanted1ivertumormodelswereestablishedinWistarratswhichwerethenrandomlyassignedintotwogroups：theshamoperationgroupinwhichtheratsonlyunderwentlaparot

6、omy，andthetreatmentgroupinwhichHIF一1QshRNAwasdeliveredintothetumorwithultrasound-targetedmicrobubbledestruction(UTMD)．Contrast—en—hancedultrasoundwasusedtorecordthetumorsize3，7，and14daysaftersonication．ThemRNA1eve1ofHIF一1awasdetectedbyqPCR，andWesternblotandimm

7、unohistochemistrywereusedtomeasuretheproteinleveIofHIF_1∞ResultsTherewasnosignificantdifferenceinthetumorsizebeforethesonicationbetweenthetwogroups．At3days．thetumorenlargedinbothgroups(P

8、einsizeinthetreatmentgroup(P<0．05)．At14days，however，thetumorbegantoshrinkinthetreatmentgroupwhileitstillenlargedintheshamop—erationgroup(P<0．05)．The1evelsofHIF_1dmRNAandproteinwere