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时间:2020-04-12
《采卵方法对西施舌早期发育生长的影响-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、特产研究SpecialWildEconomicAnimalandPlantResearch9文章编号:1001—4721(2014)03—0009—04采卵方法对西施舌早期发育生长的影响刘德经,朱善央(1.长乐市漳港海蚌场,福州350200;2.中国水产科学研究院东海水产研究所,上海20O090)摘要:采取自然排放(A组)、解剖取卵人工受精(B组)、阴干加流水刺激催产(C组)3种处理方法,将所获得西施舌的受精卵发育成直线铰合幼虫,以微藻为饵料,在室内条件下,幼虫放养密度0.2ind/mL,经9d~12d培养,观
2、察其发育生长速度、成活率及变态率。试验结果表明,A组、B组、c组9日龄幼虫平均壳长272.00tan±18.04tma、239.60tma±19.97tma、260.00tma-i-20.07tan。日平均壳长生长速度21.11treed、17.51V-,n/d、19.78tma/d。平均壳长A组与c组、C组与B组差异显著(F=9.06,P<0.O1,n=30),A组、C组与B组差异极显著(P<0.01);A组、B组、c组9日龄幼虫成活率50.00%±4.82%、37.00%士6.64%、42.86%±3.57
3、%(F=6.00,P<0.05,n=3),A组与c组差异不显著(P>0.05),A组、B组差异极显著(P<0.01);A组、B组、c组幼虫变态率51.33±6.05%、35.77±10.55%、40.59±3.19%(F=8.58,P<0.05,n=3),A组、B组差异极显著(P<0.01),A组、c组差异显著(P<0.05)。关键词:西施舌;采卵方法;幼虫发育生长中图分类号:$966.22文献标识码:AEfectsofOvnmMethodonEarlyGrowthandDevelopmentofCoeloma
4、ctraantiquateLIUDe—jing,ZHUShan—yang2(1.ChangleZhanggangSeaClamBreedingFarm,Fuzhou350200,China;2.EastChinaSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Shanghai200090,China)Abstract:Takethenaturalemissions(A),Anatomyofthethreemethodsofoocyter
5、etrievalandartificialinsemination(B),edandwatertostimulateoxytocin(C).aofstraighthingeweredevelopedforobtainingfertilizedeggsofCoelomactraantiquate.Usemicro—algaeasbaits,underlaboratoryconditions,larvalculturedensityof0.2ind/mLundertheconditionofcultureafter
6、9d一12d,guanobservedthedevelopmentoft~rowthrateandsurvivalrateandmetamorphosisrate.Resultsofexperiment,A,B,Cgroup9一day—oldlarvaeoftheaverageshelllengthwere272.0Own±18.04tan,239.60tma±19.97tma,260.00tma±20.07ttm,shellengthgrowthratewere21.11/m~/d,17.51t~n/d,19
7、.78/an/d.TherawlengIhdiferencesweresignificantforthedeterminationofresults.AandCandB,theaverageshellengthdiferenceof2.03timesand3,94times.Respectivelymistakenformachine,asignificantdiference.AnalysisvarianceF=9.06,(P<0.01,n=3O),Muld#ecompal'iflonsAandCandB,h
8、avealreadyreachedthe1%significanceleve1.(2)Respectively50.0o%±4.82%,37.00%±6.64%,42.86%±3.57%0fA,B,C9一day—oldlarvaesurvivalrate(%).AnalysisofvarianceF=6.00(P<0.05,n=3).diferencesignificant.Multi
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