英文文献汇报.pptx

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1、IF=2.2181.IntroductionMerozoitesurfaceprotein-1ofmalariaparasites,presentonthesurfaceofmerozoiteandplayingakeyroleintheinvasionprocess,isoneoftheleadingvaccinetargetsforbothP.falciparumandP.vivaxmalaria.MSP-1occursasa200kDaprecursorandundergoesstep-wiseproteolyticprocessingresultinginaglycosyl

2、-phosphatidylinositol-anchored42kDaproteinfragment(MSP-142)onthesurfaceoffreemerozoite.AlargenumberofstudieshaveshowntheprotectivepotentialofMSP-1fragments(42and19kDa)ofP.falci-parumandP.yoelii.DNAvaccineshaveemergedasapromisingapproachforavarietyofinfectiousagentsincludingmalariaparasites.DNA

3、basedvaccinesareparticularlyimportantfordevelopingmultistage/multiantigenvaccinesforcomplexparasiteslikemalariatherebyinducingspecificandprotectiveimmunityagainstdifferentlifecyclestages(pre-erythrocytic,erythrocyticandsexualstages)ofmalaria.TheadvantagesofDNAvaccinesaretheeaseoftheirproductio

4、nandtheirabilitytoinduceresponseswithoutanyexogenousadjuvant,whichisanabsoluterequirementforproteinbasedvaccineformulations.DNAvaccineshavebeenshowntoinducestronghumoralandcellularresponsestomalarialantigensinimmunized.However,clinicaltrialsofthesecandidatevaccineswhenusedaloneorinrepeatedhomo

5、logousboostingregimenshavebeendisappointing,withshortlivedlowlevelsofinducedspecificT-cellsresponses.Sequentialimmunizationwithdifferentplasmids/vectorsknownasheterologousprime-boostingappearstobeabetterapproachandhasbeenshowntoinduceenhancedandpersistentlevelsofantibodyandcellmediatedimmunere

6、sponsesagainstmalaria.Inheterologousprime-boostingstrategytheprimingwasdonewithDNAfollowedbytheboosterimmunizationwitharecombinantprotein,arecombinantviralvaccine,orexposuretotheliveorganism.InthepresentstudywehaveevaluatedtheimmunogenicityofaDNAvaccineencodingMSP-142ofP.vivaxusingprime-boosti

7、ngstrategyinBALB/cmice.WehavealsocomparedtheimmunogenicityofPvMSP-142recombinantproteininBALB/cmice.2.MaterialsandmethodsDNAvaccineconstructsencodingP.vivaxMSP-142InvitrotransfectionofmammaliancellswithP.vivaxMSP-142–pcDNA3.1plasmidMono