HyperREZ XP Carbohydrate 色谱柱维护保养手册(中文).pdf

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1、HyperREZXPCarbohydrateHyperREZXPCarbohydratecolumnsareligandexclusionphasesdesignedfortheseparationofcarbohydrates/sugarsandacids.HyperREZXPCarbohydrateCaisdesignedfortheseparationofavarietyofsugars.HyperREZXPCarbohydratePbisalsoasugarcolumnbutcanprovidealternativeselectivityandisusedwheretheHyperR

2、EZXPcarbohydrateCaphaseprovideslimitedornoresolutionoftwospecificsugars.HyperREZXPNaistheidealchoicefortheseparationofpolysaccharides.HyperREZXPcarbohydrateHissuitablefortheseparationofsugars,butalsoshowsexcellentseparationoforganicacids.ThetablebelowprovidesasimplemethodofselectingasuitableHyperRE

3、ZXPCarbohydratecolumnforspecificsugars.Column:300x7.7mm,mobilephase:water,flowrate:0.6ml/min,detection:RI,temperature:75°C(H),85°C(Ca),80°C(Pb)SaccharideRetentionTime/minHCaPbAldonitol11.514.920.4Arabinose11.413.619.4Erythritol12.715.620.3Fructose10.613.519.3Fucose12.213.717.1Galactose1.112.215.6Gl

4、ucose9.911.113.9Glycerol14.116.119.5Lactose8.69.712.8Maltose8.49.512.5Maltotriose7.78.711.9Mannitol11.017.328.9Mannose1.512.516.7Raffinose8.28.611.4Sorbitol11.120.7naSucrose9.89.411.9Xylose10.612.015.0Stability稳定性CounterIonCaPbHNaMaxflow1.21.21.20.6rate最大流-1速/ml.minTypicalflow0.60.60.60.3rate典型流-1速

5、.ml/minMax69bar69bar6941barpressure最bar大耐压Typical<24bar<24bar<24b<14barpressure典ar型压力Typical85856585operating典型使用温度temp./°CMobile<30%acetonitrileor<5%alcoholphase流动<30%乙腈或<5%乙醇相ConditioningThecolumnshouldberunat0.1ml/minuntiltherequiredoperatingtemperatureisreached(approx.80°C).Havingachievedthereq

6、uiredtemperature,theflowshouldbegraduallyincreaseduntilthetestflowrateisachieved.色谱柱先用0.1ml/min流速，到温度升至实验温度（如80°C），然后开始提高流速到实验需要的流速。StorageThecolumnshouldideallybekeptattheoperatingtemperaturewithaflowof0.1ml/min.Forlongertermstoragetheflowshouldbeslowlyreducedto0.1ml/minandtheheatingswitchedoff.Ha

7、vingreachedambienttemperatureitisrecommendedthatthecolumnbestoredintheaqueousmobilephasewiththeend-capssecurelyfittedandplacedinarefrigeratordownto4°Cwithcaretakennottofreeze.先将流速下降到0.1ml/min，然后降温到常温，关掉柱温箱。