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1、14中国肿瘤临床与康复 2002年第9卷 第2期·论著·恶性血液病治疗前后T细胞亚群和NK细胞活性测定分析陆紫敏,顾凤英,申远,吴金莺(同济大学附属同济医院、血液内科、肿瘤实验室.上海200065)[摘要]目的 检测46例恶性血液病患者化疗前后外周血T细胞亚群及NK细胞活性,观察胸腺肽免疫功能。方法 应用单克隆抗体和流式细胞仪。结果(1)恶性血液病患者各项免疫活性细胞表达均低于对照组;(2)晚期和复发型恶性血液病患者各项免疫指标均低于初发型恶性血液病;(3)AL各项免疫指标明显低于其它恶性血液病;(4)治疗后A、B两组
2、比较;A组的NK细胞活性明显高于B组,A组治疗后CD3、CD4、CD4/CD8、NK细胞活性明显高于治疗前。结论T细胞亚群、NK细胞活性在恶性血液病患者中受到抑制并随病情程度而进一步加剧,其中AL患者受抑更甚,胸腺肽能明显提高恶性血液病患者的细胞免疫功能,尤其对NK细胞,它是一种低毒高效的生物反应调节剂。[关键词] 恶性血液病:T细胞亚群;NK细胞活性;胸腺肽[中图分类号]R733.73[文献标识码]A[文章编号]1005-8664(2002)02-0014-03DeterminationofTcellsubsetsa
3、ndNKcellactivitybeforeandaftertreatmentinmalignanthemopathicpatientsLUZi2min,GUFeng2ying,SHENYuan,etal.AffiliatedTongjiHospitalofShanghaiTongjiUniversity,Shanghai200065,China[Abstract]ObjectiveTodeterminetheperipheralbloodTcellandNKcellactivityin46casesofmaligna
4、nthemopathicpatientsbeforeandafterchemotherapy,andtoobservethymopeptides(TP)immunefunction.MethodsTheactivitiesofpe2ripheralbloodTcellsubsetsandNKcellweredeterminedwithmonoclonalantibodyandflowcytometry.Results(1)Theim2muneindexesofmalignanthemopathicpatientswer
5、elowerthanthoseofthecontrolgroup.(2)Theimmuneindexesofthead2vancedandrecurrentmalignanthemopathicpatientswerelowerthanthoseoffirstoccurredpatients.(3)Theimmuneindexesoftheacuteleukemiawereobviouslylowerthanthoseofanyothermalignanthemopathy.(4)Aftertreatment,theN
6、Kcellactivity++inAgroupwassignificantlygreaterthanthatofBgroup.TherewasastatisticallysignificantincreaseinCD3andCD4cells,ra2++tioofCD4/CD8andNKcellactivityaftertreatment.ConclusionTheactivityofTcellsubsetsandNKcellinmalignanthemopathicpatientswasfurtherinhibited
7、withtheadvancingofillness.Thesuppressiononacuteleukemiapatientsismoresig2nificant.Thethymopeptidescansignificantlyimprovethecellimmunefunctionofmalignanthemopathicpatients,particularlyforNKcell.Itisahighlyeffectivebiological2response2regulatorwithfewsideeffects.
8、[Keywords]malignanthemopathy;Tcellsubsets;NKcellactivity;thymopeptidesT淋巴细胞亚群作为机体免疫防御体系重要部52岁。均经骨髓细胞学、组化染色、生化或病理活检分,而NK细胞作为一种天然免疫活性细胞,在肿瘤的确诊。本组急性白血病(AL)18例,多发性骨髓瘤发生发展
